Skip to main content
. 2021 Dec 18;25(1):103643. doi: 10.1016/j.isci.2021.103643

Figure 3.

Figure 3

Surface expression and subcellular distribution of α2-ARs and their mutants in HEK293 and SHSY5Y cells

(A) The expression of α2B-AR and its mutants in HEK293 and SHSY5Y cells. The cells were transfected with α2B-AR or individual mutants and their expression was measured by radioligand binding of intact live cells. The data are expressed as percentages of WT. Bars represent mean ± SE (n = 3). One-way ANOVA test; ∗p < 0.05 versus WT.

(B) Subcellular distribution of α2B-AR and its mutants in HEK293 and SHSY5Y cells revealed by confocal microscopy. The images shown are representatives of 3 experiments. Scale bar, 10 μm.

(C) Colocalization of the YS-AA mutant of α2B-AR with giantin in HEK293 and SHSY5Y cells. Scale bar, 10 μm.

(D) Colocalization of the YS-AA mutant of α2A-AR with giantin in HEK293 and SHSY5Y cells. Scale bar, 10 μm.

(E) Quantitative data shown in (C) and (D). The data are expressed as percentages of total expression with a total of at least 20 cells quantified in each experiment. Bars represent mean ± SE (n = 3). Unpaired Student's t test; ∗p < 0.001 versus HEK293 cells.