REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-GM130 (clone 35)	BD Biosciences	610823
Rabbit polyclonal anti-giantin	Abcam	ab80864
Goat anti-rabbit IgG (H+L), Alexa Fluor 594	Thermo Fisher Scientific	A-11012
Goat anti-mouse IgG (H+L), Alexa Fluor 594	Thermo Fisher Scientific	A-11032
Chemicals, peptides, and recombinant proteins
[3H]-RX821002	Perkin-Elmer	NET1153250UC
[3H]-CGP12177	Perkin-Elmer	NET1061250UC
[N-methyl-3H]-scopolamine methyl chloride	Perkin-Elmer	NET636250UC
Lipofectamine 2000	Thermo Fisher Scientific	11668019
Dulbecco's modified eagles medium	HyClone	SH30243.01HI
Ham's F-12 nutrient mix	Thermo Fisher Scientific	11765054
Minimum essential medium	Thermo Fisher Scientific	11095080
Neurobasal-A medium	Thermo Fisher Scientific	12349015
B-27 supplement	Thermo Fisher Scientific	17504001
GlutaMax supplement	Thermo Fisher Scientific	35050061
Fetal bovine serum	HyClone	SH30396.03HI
Penicillin-streptomycin solution	HyClone	SV30010
ProLong gold antifade mountant with DAPI	Invitrogen	P36931
Experimental models: Cell lines
HEK293	ATCC	CRL-1573
SHSY5Y	ATCC	CRL-2266
Sprague-Dawley rats	Charles River Laboratories
Primers (Table S1)	This paper	N/A
Recombinant DNA
α2B-AR-GFP	(Wu et al., 2003)	N/A
α2B-AR YS-AA-GFP	(Dong and Wu, 2006)	N/A
α2B-AR L48-A-GFP	(Duvernay et al., 2009a)	N/A
α2B-AR F436-A-GFP	(Duvernay et al., 2004)	N/A
α2B-AR IL-AA-GFP	(Duvernay et al., 2004)	N/A
β2-AR-GFP	(Wu et al., 2003)	N/A
β2-AR LL-AA-GFP	(Duvernay et al., 2009a)	N/A
β2ARα2ct-GFP	(Dong et al., 2010a)	N/A
β2ARα2ct IL-AA-GFP	This paper	N/A
M3R-GFP	(Wei et al., 2021)	N/A
M3R LL-AA-GFP	This paper	N/A
DsRed	Clontech Laboratories	632466
VSVG-GFP	Addgene	11912
VSVG DxE-AxA-GFP	This paper	N/A
VSVGct-GFP	(Li et al., 2017)	N/A
VSVGct LL-AA-GFP	This paper	N/A
VSVGct DxE-AxA-GFP	This paper	N/A
VSVGct DxE-AxA/LL-AA-GFP	This paper	N/A
α2A-AR-GFP	(Li et al., 2017)	N/A
α2A-AR YS-AA-GFP	(Dong and Wu, 2006)	N/A
DsRed2-ER	(Dong and Wu, 2006)	N/A
Software and algorithms
ImageJ	NIH	imagej.nih.gov/ij/