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. 2022 Jan 3;10:e12263. doi: 10.7717/peerj.12263

Figure 4. miR-100-5p hampers cell malignant behaviors by targeting CDC25A in BC.

Figure 4

The (A) mRNA and (B) protein levels of CDC25A in transfected cells assayed through qRT-PCR and western blot; (C) Proliferation, (D) clonogenic, (E) migration and (F) invasion properties of MCF-7 cells in different treatment groups assayed through CCK8, clonogenic , wound healing (40×) and Transwell assays (100×), and (G) flow cytometry was carried out for determination of cell apoptotic rate; * P < 0.05; Each experiment was repeated three times, including three technical replicates and three biological replicates, and representative figures were selected.