Abstract
Owing to the increased use of serum enzyme determinations in veterinary diagnostic work, greater knowledge about the keeping qualities of different animal sera under various storing conditions seems desirable.
The present paper deals with the stability of serum aspartate aminotransferase (AspAT = GOT), alanine aminotransferase (A1AT = GPT), lactate dehydrogenase (LDH), and a-hydroxybutyrate dehydrogenase (HBD) in cattle, sheep, and swine.
Sera from 14—16 animals of each species were analysed daily for 5 days after storage at room temperature (22°C) and in the refrigerator (4°C). Samples kept in the deep-freezer (—20°C) were reanalysed once after 32—38 days.
Significant differences of serum activity were found between individuals for all enzymes in the three species.
Great variations were found in the stability of enzyme activities of different species.
To summarize, it may be said that the changes of transferase activities were less pronounced under the different storing conditions than those of the dehydrogenases investigated. Pig serum in particular showed heavy losses of the latter enzymes already after 1 day, more pronounced at refrigerator than at room temperature.
As a consequence of the results obtained, practical recommendations for analytical work on these enzymes are suggested.
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Acknowledgments
The author wishes to thank Mr. T. W. Gedde-Dahl, Lic. Agr., for advice and assistance in the statistical work.
References
- Amelimg D, Hofmann L, Otto L. Untersuchungen zur Haltbarkeit der Ferment-Aktivität im Serum. (Investigations on the stability of enzyme activity in serum) Dtsch. med. Wschr. 1966;91:851–853. doi: 10.1055/s-0028-1110664. [DOI] [PubMed] [Google Scholar]
- Feissli S, Forster G, Laudahn G, Schmidt E, Schmidt F W. Normal-Werte und Alterung von Hauptketten-Enzymen im Serum. (Normal values and changes of main chain enzymes in serum.) Klin. Wschr. 1966;44:390–396. doi: 10.1007/BF01745931. [DOI] [PubMed] [Google Scholar]
- Hyldgaard-Jensen J. Maelkesyredehydrogenase hos svin. I. Mselke-syredehydrogenase (LDH) i blodplasma. (Lactic dehydrogenase (LDH) in pig plasma) København: Kgl. Vet.- og Landbohøjsk., Inst. Sterilitetsforskn. Aarsberetn.; 1966. pp. 117–125. [Google Scholar]
- Kreutzer H H, Fennis W H S. Lactic dehydrogenase isoenzymes in blood serum after storage at different temperatures. Clin, chim. Acta. 1964;9:64–68. doi: 10.1016/0009-8981(64)90045-2. [DOI] [PubMed] [Google Scholar]
- Raabo E. Determination of serum lactic dehydrogenase by the tetrasolium salt method. Scand. J. clin. Lab. Invest. 1963;15:233–238. doi: 10.3109/00365516309079738. [DOI] [PubMed] [Google Scholar]
- Report of the Commission on Enzymes of the International Union of Biochemistry. I.U.B. Symposium, Series 20, Pergamon Press, Oxford 1961.
- Schmidt E, Schmidt F W. Untersuchungen über die Alterung von Enzymen in vitro. (Ageing of enzymes in vitro) Enzym, biol. clin. 1963;3:80–86. doi: 10.1159/000458046. [DOI] [PubMed] [Google Scholar]
- Südhof H, Wötzel E. Über die Haltbarkeit von zur Enzymaktivitätsbestimmung vorgesehener Serumproben. (Stability of serum samples for enzyme activity determination) Klin. Wschr. 1960;38:1165–1167. doi: 10.1007/BF01492889. [DOI] [Google Scholar]
- Tollersrud S, Ribe O. Serum enzymes in pregnant ewes and their newborn lambs on a vitamin E-deficient ration. Acta vet. scand. 1967;8:1–13. [Google Scholar]
- Tollersrud, S. & Inger Nafstad: The vitamin E-deficiency syndrome in pigs. II. Investigations on serum and tissue enzyme activity. In press. [DOI] [PMC free article] [PubMed]
- Wilkinson J H. Isoenzymes. London: E. & F.N. Spon Ltd.; 1965. [Google Scholar]