Abstract
A method for rapid electrophoresis of serum proteins on a cellulose acetate membrane is described.
In order to experimentally identify the various larger globulin fractions in normal bovine serum, pure bovine y-globulin and a bovine attraction (Antitrombin III) were used.
These fractions were electrophoretically compared with bovine serum and the various fractions on the diagram determined.
A diluted veronal buffer with added Ga-acetate gave considerably better separation than the reported standard buffer.
In the last part of the experiment normal percentage values for the composition of the protein fractions in bovine serum were calculated by the method described. The material consisted of 50 cows of the N.R.F. breed. The values are compared with corresponding figures reported by other authors, and the results are discussed.
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Acknowledgment
Antitrombin III was supplied by Dr. U. Abildgaard, Ullevâl Hospital, Department IX, Oslo, who also gave valuable advice and help during the experimental work. The author owes him a great debt of gratitude.
The author would also like to thank Prof. dr. M. Braend, at present with the Faculty of Veterinary Science, University College, Nairobi, Kenya, for his valuable advice and guidance during the experiment.
References
- Abildgaard U. Highly purified antithrombin III with cofactor activity prepared by disc electrophoresis. Scand. J. clin. Lab. Invest. 1968;21:89–91. doi: 10.3109/00365516809076981. [DOI] [PubMed] [Google Scholar]
- Abildgaard, U.: Personal communications 1968b.
- Anon.: Model R paper electrophoresis system Instruction Manual. RIM-5. Beckman Instruments, Inc., Spinco Division, Palo Alto, Calif. 1957.
- Anon.: Model R-101 microzone electrophoresis cell Instruction Manual. RM-IM-3. Beckman Instruments, Inc., 2500 Harbor Blvd., Fuller-ton, Calif. 1965.
- Baumstark JS, Laffin RJ, Bardawil WA. A preparative method for the separation of 7 S gamma globulin from human serum. Arch. Biochem. 1964;108:514–522. doi: 10.1016/0003-9861(64)90436-9. [DOI] [PubMed] [Google Scholar]
- Irfan M. The electrophoretic pattern of serum proteins in normal animals. Res. vet. Sci. 1967;8:137–142. doi: 10.1016/S0034-5288(18)34626-5. [DOI] [PubMed] [Google Scholar]
- Kohn J. A cellulose acetate supporting medium for zone electrophoresis. Clin. chim. Acta. 1957;2:297–303. doi: 10.1016/0009-8981(57)90005-0. [DOI] [PubMed] [Google Scholar]
- Laboiiche C. Calcue d’erreurs dans l'interprétation quantitative des electrophoreses de sérum des bovidés domestiques. (Calculation of errors in the quantitative interpretation of electrophoresis of the serum of domestic bovines) Ann. Biol. anim. 1962;2:251–263. [Google Scholar]
- Laurell CB, Laurell G, Skoog N. Buffercomposition in paper electrophoresis. Considerations on its influence, with special reference to the interaction between small ions and proteins. Clin. Chem. 1956;2:99–111. doi: 10.1093/clinchem/2.2.99. [DOI] [PubMed] [Google Scholar]
- Perk KS, Lobi K. Chemical and electrophoretic studies of the serums of cattle infected naturally with Asia-1 type Foot-and-Mouth disease virus. Amer. J. vet. Res. 1961;22:217–223. [PubMed] [Google Scholar]
- Turba F, Enenkel HJ. Electrophoresis in filter paper. Naturwis-senschaften. 1950;37:93–99. doi: 10.1007/BF00631958. [DOI] [Google Scholar]
- Vesselinovitch SD. The analysis of serum proteins of domestic animals by filter-paper electrophoresis. A review. Cornell Vet. 1959;49:82–96. [PubMed] [Google Scholar]