Abstract
The sensitivity and error limits of a conventional in ovo neutralization test for antibody to distemper virus were compared with the same parameters of a single dilution-probit test for neutralizing antibody.
With the former test, antibody was demonstrable in ferrets 8 days following a single subcutaneous injection of virus while probit analysis of data from the single dilution test revealed the appearance of antibody as early as 4 days post-immunization. The error terms of the single dilution test were relatively large as compared to those of the conventional test for antibody.
Distinctly biphasic antibody responses were observed in ferrets following a single subcutaneous injection of distemper virus.
Abstract
Die Empfindlichkeit samt Standardfehlern und Sicherheitsgrenzen bei einem konventionellen in ovo Neutralisationstest für Antikörper gegen Hundestaupevirus wurden mit ensprechenden Eigenschaften bei einem s.g. „einzigen Verdünnungs”-probit Test für neutralisierende Antikörper verglichen.
Mit den konventionellen Testen konnten Antikörper bei Fretten 8 Tage nach einer subkutanen Virusinjektion entdeckt werden. Probit Analyse von Resultaten von „einzigen Verdünnungs” Testen enthüllte dagegen das Auftreten von Antikörpern bereits 4 Tage nach Immunisierung. Der Standardfehler in „einzigen Verdünnungs” Testen war relativ gross verglichen mit dem in den konventionellen Testen für Antikörper.
Deutlich bifasische Antikörperantworten wurden bei Fretten nach einer einzigen subkutanen Injektion von Hundestaupevirus erhalten.
Sammanfattning
Känsligheten samt standardfei och trygghetsgränser hos en konventionell in ovo neutralisationstest för antikroppar mot valpsjukevirus jämfördes med motsvarande egenskaper hos en s.k. „enda spädning”-probit test för neutraliserande antikroppar.
Med den konventionella testen kunde antikroppar hos fretter upptäckas 8 dagar efter en subkutan virusinjektion; Probit analys av resultat från „enda spädning” testen avslöjade däremot uppträdandet av antikroppar redan 4 dagar efter immunisering. Standardfeiet i „enda spädning” testen var relativt stort jämfört med det i den konventionella testen för antikroppar.
Tydligt bifasiska antikroppssvar erhölls hos fretter efter en enda subkutan injektion av valpsjukevirus.
Full Text
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Footnotes
Abstract of portion of author’s Master of Science thesis, Washington State University, 1963.
To whom requests should be addressed
Washington Agricultural Experiment Station, Scientific Paper No. 2593.
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