Figure 1.

NR supplementation alters the plasma and muscle NAD metabolome in DIO CrAT-deficient and control mice

(A) Prevailing view whereby oral NR administration impacts skeletal muscle metabolism.

(B) Intervention cohort experimental design in CrAT^fl/fl (floxed control, FC) and CrAT^MCK (knock out, KO) mice.

(C) Plasma NR.

(D) Plasma NAM.

(E) Skeletal muscle NAD.

(F) Skeletal muscle NADH.

(G) Skeletal muscle NAM.

(H) Skeletal muscle NMN. Data are represented as mean ± SEM.

(C–H) N = 5–8 per group. Data were analyzed by two-way ANOVA. ∗ represents a main effect of treatment, # represents a main effect of genotype, and ★ represents an interaction between treatment and genotype. ∗P≤0.05. N represents biological replicates.

See also Figure S1 and Table S2.