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. 2022 Jan 9;14(1):2013594. doi: 10.1080/19420862.2021.2013594

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Purity analysis by SE-HPLC and SDS-PAGE of c4G7. (a) SE-HPLC chromatogram of final product. (b) Reducing and non-reducing SDS-PAGE for final product from 3 batches of c4G7. Lane 1: reduced mAb standard, lane 2–3: blank, lane 4: reduced c4G7 batch 1, lane 5: reduced c4G7 batch 2, lane 6: reduced c4G7 batch 3, lane 7: blank, lane 8: reduced mAb standard, lane 9: molecular weight standards, lane 10: non-reduced mAb standard, lane 11: blank, lane 12: non-reduced c4G7 batch 1, lane 13: non-reduced c4G7 batch 2, lane 14: non-reduced c4G7 batch 3. SEC-HPLC analysis of c4G7 final product. Main peak indicates monomeric product. Other peaks observed are sample buffer related. 4b. Reduced and Non-Reduced SDS-Page analysis of three different batches of c4G7. Each sample matches the mAb standard indicating conformity.