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. 2022 Jan 4;14(1):217. doi: 10.3390/nu14010217

Figure 4.

Figure 4

IBF-R determines adipose tissue expansion and adipogenic factors in eWAT. (a) H&E staining in eWAT. Scale bar = 50 µm. (b) The mean diameter of adipocytes in eWAT. (c–f) C/EBPα, PPAR-γ, FAS and SREBP-1c were measured in eWAT by qRT-PCR. (g) Immunoblotting of PPAR-γ, C/EBPα, SREBP-1c, FAS, and β-actin expressions in eWAT. (h–k) Quantification of protein expressions. (l,m) Immunoblotting of p-AMPK, AMPK, SIRT-1, and β-actin expressions in eWAT and respective quantitative analysis. Data are shown as mean ± SEM (n = 10, * p < 0.05 vs. NCD + vehicle, # p < 0.05 vs. HFD + vehicle). eWAT; epididymal white adipose tissue.