TABLE 1.
Mutations affecting trypanosome mitochondrial functions that were assessed for their effects on tsetse infectivity in this studya
| Gene and mutation (TriTrypDB gene ID) | Expected consequence of mutation(s) | References |
|---|---|---|
| kDNA0 (NA) | Complete absence of essential subunits of respiratory complexes I, III, IV, and of the Fo part of complex V. Note that kDNA0 can be tested only in combination with a compensatory mutation, in this study the F1-γ L262P. | GenBank entry MK584625; 66 |
| F1 subunit γ (Tb927.10.180), heterozygous or homozygous L262P mutation (WT/L262Pγ; L262P/L262Pγ) | F1-ATPase reaction: appears to lower Km for ATP and increase rate of ATP hydrolysis; F1Fo-ATP synthase reaction: (partial?) uncoupling of Fo-γ rotation and F1 activity, resulting in complete or partial loss of OXPHOS in homozygous mutant | 17, 66, 84, 85, 88, 89 |
| Fo subunit Tb1 (Tb927.10.520), null mutant (Tb1−/Tb1−) | Loss of F1Fo-ATP synthase complex, accumulation of F1, resulting in complete loss of OXPHOS | 107, 109 |
a
See Table S1 for details on the corresponding cell lines. NA, not applicable.