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. 2022 Jan 5;11(1):173. doi: 10.3390/cells11010173

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Characteristics of sulfation patterns and glucuronosyl epimerization levels in the tested variants of dermatan sulfate (DS). (A) Comparison of the disaccharide profiles that were obtained after the tested variants of DS were treated with chondroitinase ABC. The relative content of an individual disaccharide was calculated as a percentage contribution of the area under its peak into the total area of the chromatogram. Chromatographical analysis of sulfation pattern in DS from the porcine intestinal mucosa (PM) was conducted previously [17]. The results are expressed as the mean of two independent experiments. (B) Comparison of the glucuronosyl epimerization levels that characterized the tested variants of DS. This parameter was calculated as the ratio between the total areas under the peaks of the unsaturated disaccharides that were released by chondroitinase AC I and by chondroitinase ABC. The results are expressed as the mean ± SD of two independent experiments. PS—DS from porcine skin, NF—DS from the normal human fascia, DF—DS from fibrosis affected fascia.