Figure 6. Binding of monoclonal antibodies (mAbs) to S. aureus clinical isolate biofilm.

(A) Biofilm of clinical isolates derived from catheter tip, endocarditis, and prosthetic joint infections (PJIs) was grown for 24 hr and incubated with 33 nM F598-IgG3. mAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader. (B) Scatter plot of F598-IgG3 binding to isolates and biofilm adherent biomass measured by crystal violet staining after mAb binding assay. Isolates selected for (C) are indicated. (C) Biofilms of clinical isolates was grown for 24 hr and incubated with 33 nM IgG3 mAbs. mAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader. Data (A) represent mean + SD of three independent experiments. One-way ANOVA followed by Dunnett test was performed to test for differences in antibody binding versus LAC KO and displayed only when significant as *. Exact p-values are displayed in Supplementary file 2. Data (B) represent mean two independent experiments.