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. 2022 Jan 5;9(1):ENEURO.0481-21.2021. doi: 10.1523/ENEURO.0481-21.2021

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Copyright © 2022 Souter et al.

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Figure 3. — VGLUT2-expressing projections from MHb to IPN. A, Fluorescent in situ hybridization from Kiaa^Cre mouse showing Cre and Slc17a6 expression in the MHb (outlined); scale bar: 100 μm. B, High-resolution image showing expression of Cre, Slc17a6 (VGLUT2), and DAPI in MHb of Kiaa^Cre mouse; scale bar: 10 μm. Yellow arrows indicate some of the cells containing both Cre and Slc17a6 mRNA. C, Image of MHb from Slc17a6^Cre (VGLUT2) mouse injected with AAV1-Ef1α-DIO-ChR2:mCherry bilaterally into the MHb (outlined); scale bar: 100 μm. D, Immunohistochemistry of IPN from Slc17a6^Cre (VGLUT2-Cre) mouse injected bilaterally with AAV1-Ef1α-DIO-ChR2:mCherry in MHb. VGLUT2^Cre MHb terminals in IPN represented by mCherry expression, stained with VGLUT1 and ChAT; scale bar: 100 μm.