FIG 6.

Influence of CofD/FbiA on the biosynthesis of F₄₂₀-0 derivatives. (A) CofC from M. rhizoxinica, (B) CofC from Mycetohabitans sp. B3, and (C) FbiD from M. smegmatis, as well as (D) Ca. E. factor, (E) Ca. H. archaeon, and (F) Archaeon GBE54128 were tested with either M. jannaschii CofD (standard assay) or together with their cognate CofD enzyme from the same source organism. (G) Schematic drawing illustrating the channeling of the labile metabolites LPPG, EPPG, GPPG from CofC to CofD. While CofC selects primary substrates, CofD is able to select between these intermediates. For abbreviations see Fig. 1.