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. 2022 Jan 18;204(1):e00520-21. doi: 10.1128/JB.00520-21

Second Correction for Singh et al., “Requirement of the mymA Operon for Appropriate Cell Wall Ultrastructure and Persistence of Mycobacterium tuberculosis in the Spleens of Guinea Pigs”

Amit Singh 1, Radhika Gupta 1, R A Vishwakarma 2, P R Narayanan 3, C N Paramasivan 3, V D Ramanathan 3, Anil K Tyagi 1,
PMCID: PMC8765390  PMID: 35040687

AUTHOR CORRECTION

Volume 187, no. 12, p. 4173–4186, 2005, https://doi.org/10.1128/JB.187.12.4173-4186.2005, and volume 203, no. 10, e00094-21, 2021, https://doi.org/10.1128/JB.00094-21. We acknowledge the following inadvertent errors which came to light after the previous correction was published.

Page 4175, Fig. 1C: There is undeclared splicing between lanes 4 and 5.

Page 4177, Fig. 4: There are inadvertent duplications in both panels. The Mtbmym::hyg and MtbΔvirS_virS lanes are the mirror images of the Mtb and MtbΔvirS lanes. The FAMEs and MAMEs bands were detected on preparative thin-layer chromatography plates, quantified in a phosphorimager, and further processed in Adobe Photoshop. For each experiment, numerous images of different exposure durations were generated and processed. The inadvertent errors occurred during the assembly for the preparation of final figures. We no longer have the raw images, due to the age of the article and subsequent retirement of the corresponding author. The data shown in Fig. 4 were also independently validated in this paper by using high-resolution analytical techniques such as high-pressure liquid chromatography (Fig. 3), matrix-assisted desorption ionization–time of flight analysis (Fig. 5), and gas chromatography (Fig. 6 and 7).

These inadvertent errors do not change the scientific conclusions of the study in any manner. We apologize to the journal readers for these unintentional errors.


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