Figure 3.

Chemical approaches to enrich modified sites in a complex RNA pool. a) Schematic of chemical probing of RNA structure and enrichment through the use of bi-functional probing reagents. b) Chemical structure of NAI-N₃, a bi-functional reagent used for icSHAPE probing. c) Chemical structure of biotinylation reaction with NAI-N₃ and DBCO-biotin for biotinylation of icSHAPE adduct sites. d) Chemical structure of NAz-N₃, a bi-functional reagent used for icLASER probing. e) Chemical structure of biotinylation reaction with NAz-N₃ and with DBCO-biotin for biotinylation of icLASER adduct sites. f) Chemical structure of kethoxal-N₃, a bi-functional reagent used for icLASER probing. g) Chemical structure of biotinylation reaction with kethoxal-N₃ and DBCO-biotin for biotinylation of keth-seq adduct sites.