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. 2022 Jan 6;10(1):120. doi: 10.3390/biomedicines10010120

Figure 5.

Figure 5

Ablation of TRPM5 abolishes the effects of saccharin in mouse islets and in vivo. (A) Representative traces of intracellular calcium response in WT and TRPM5-KO primary beta-cells treated (vertical line) with saccharin (25.0 mM) in the presence of 8.3 mM glucose (n = 3 mice per group; average of 5–10 cells). Insets: calcium response shown as change between baseline and peak calcium response. Area under curve (AUC) calculated as response X time. Two-tailed unpaired T-test. (B) Static insulin release in response to saccharin (20 mM) in the presence of 8.3 mM glucose in WT and TRPM5-KO islets (n = 6 mice per group). Data are expressed as fold change from 8.3 mM glucose (set at value 1) using paired experiments. Two-tailed unpaired T-test. (C) Plasma insulin in response to a bolus of saccharin (sac; 1.0 g/kg) or saline (sal) injected in WT and TRPM5-KO (n = 5 mice per group). Area under curve (AUC) calculated between 0 to 10 min. ANOVA Fisher’s LSD post hoc.