Table 3.

Effect of time of incubation (“Time”), sperm status (“Status”: hyperactivated vs. non-hyperactivated), and interactions between time and status (“Interaction”) on kinetic parameters in three mouse species (M. musculus, M., spretus, and M. spicilegus) incubated under capacitating conditions (mT-BH medium). Values F and p correspond to statistical analyses of repeated measure ANOVAs. Individual was used as a random factor, and incubation conditions and time were used as fixed factors. Results in bold indicate significant differences (p < 0.05) in statistical analyses. VCL: curvilinear velocity; VSL: straight-line velocity; LIN: linearity; ALH: amplitude of lateral head displacement; BCF: beat-cross frequency.

Dependent Variable	Independent Variable	Mus musculus		M. spretus		M. spicilegus
		F	p	F	p	F	p
VCL	Status	215.217	<0.001	59.995	<0.001	1.507	0.223
	Time	1.856	0.142	0.921	0.433	5.03	0.003
	Interaction	0.376	0.771	0.773	0.511	5.361	0.002
VSL	Status	3.589	0.061	37.789	<0.001	111.676	<0.001
	Time	9.063	<0.001	8.13	<0.001	16.04	<0.001
	Interaction	0.279	0.841	0.303	0.823	0.771	0.513
LIN	Status	20.994	<0.001	125.778	<0.001	179.422	<0.001
	Time	13.285	<0.001	17.541	<0.001	17.741	<0.001
	Interaction	0.15	0.929	0.519	0.670	0.603	0.698
ALH	Status	2409.089	<0.001	2132.668	<0.001	2254.932	<0.001
	Time	1.792	0.154	5.758	0.001	30.844	<0.001
	Interaction	0.726	0.539	6.662	<0.001	0.129	0.943
BCF	Status	8.341	0.005	63.394	<0.001	215.774	<0.001
	Time	0.168	0.917	37.874	<0.001	4.18	0.008
	Interaction	0.268	0.849	0.018	0.997	1.772	0.128