Figure 3.

The CDK inhibitors increase the interaction between PXR, DNAJC7 and Hsp90. HepG2 cells were treated with kenpaullone (5 μM) (A) or dinaciclib (4 μM) (B) for 24 h, and the interactions of PXR with DNAJC7 and Hsp90 were investigated by co-immunoprecipitation. Cell lysates were immunoprecipitated with anti-PXR antibody, anti-Hsp90 antibody or anti-DNAJC7 antibody, followed by immunoblotting with anti-PXR antibody, anti-Hsp90 antibody or anti-DNAJC7 antibody, respectively. Experiments described in this figure were repeated independently at least three times.