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Suppressing glycogen synthesis and breakdown influences carbohydrate levels: glycogen and trehalose content in wild-type and daf-2(e1370) mutant treated with gsy-1 (A,B) and pygl-1 (C,D) RNAi, respectively. Error bars indicate SEM of three independent replicates. EV—empty vector control. ns: p > 0.05, * p ≤ 0.05, **** p ≤ 0.0001 (interaction, two-way ANOVA). The data are summarized in Table S2.
