Table 2.
List of identified proteins previously described in ocular surface disease. The mean spectral count and standard deviation (SD) of each protein in the whole strip (W), the bulb (B), and the rest (R) of the strip are presented. The proteins are classified as those common in all batches (1), two batches (2), or one batch (3) and are ranked according to their mean of spectra in W. *, proteins reported in dry eye disease studies. Accession number refers to UniProt identification.
| Accession Number | Protein Name | W | B | R |
|---|---|---|---|---|
| Mean ± SD | ||||
| (1) Common in all batches | ||||
| P12273 | Prolactin-induced protein [1] | 142.3 ± 14 | 108 ± 15.4 | 121.7 ± 2.5 |
| P98160 | Heparan sulfate proteoglycan 2 [1,29] | 113.3 ± 5.5 | 56.3 ± 4.9 | 123.7 ± 1.5 |
| P61626 | Lysozyme C * [6,29,44] | 111.3 ± 6.7 | 104 ± 4.6 | 108.7 ± 5.5 |
| P01876 | Ig alpha-1 chain C region [6,29] | 107 ± 2.6 | 61.7 ± 3.2 | 74 ± 4.4 |
| P02768 | Serum albumin * [6,45] | 101.7 ± 2.1 | 204.3 ± 8.1 | 67 ± 4 |
| P02788 | Lactotransferrin * [29,44] | 100.7 ± 5.6 | 130.3 ± 7.1 | 101.7 ± 3.5 |
| P01024 | Complement C3 [22,44] | 72.7 ± 3.2 | 69.7 ± 1.5 | 62.7 ± 2.1 |
| Q9UGM3 | Salivary agglutinin [1,44] | 50.7 ± 4 | 48 ± 2 | 54.3 ± 0.6 |
| P31025 | Lipocalin-1 * [6,29,44] | 45.3 ± 2.1 | 50 ± 3.6 | 36.7 ± 2.3 |
| P00352 | Retinal dehydrogenase 1 [23,44] | 35.3 ± 2.1 | 46 ± 4 | 24.7 ± 2.1 |
| O75556 | Mammaglobin-B [1,44] | 35.3 ± 0.6 | 18 ± 1 | 6.3 ± 1.2 |
| P06733 | Alpha-enolase [1,44] | 31.3 ± 1.2 | 52 ± 4.6 | 27.3 ± 0.6 |
| P02787 | Serotransferrin [46] | 24.7 ± 3.1 | 59 ± 4 | 26.7 ± 1.5 |
| P07900 | Heat shock protein HSP 90-alpha [23,47] | 22.3 ± 1.5 | 8 ± 2.6 | 24.3 ± 1.5 |
| P04083 | Annexin A1 [23,44] | 22.3 ± 0.6 | 24.7 ± 0.6 | 18 ± 1 |
| P06702 | Protein S100-A9 [1] | 19 ± 1 | 28.7 ± 2.1 | 22 ± 1 |
| P07858 | Cathepsin B [1] | 18.3 ± 1.5 | 19.7 ± 1.5 | 22 ± 1.7 |
| P47895 | Aldehyde dehydrogenase family 1 member A3 [23,48] | 18 ± 2.6 | 23.7 ± 1.5 | 6.3 ± 2.1 |
| P00738 | Haptoglobin [1] | 18 ± 2 | 19 ± 1.7 | 14 ± 0 |
| P30740 | Serpin B1 [22,23] | 15.7 ± 2.1 | 24.3 ± 1.2 | 8.7 ± 3.2 |
| P05109 | Protein S100-A8 [1] | 15.7 ± 0.6 | 22.3 ± 3.2 | 22 ± 2.6 |
| P25311 | Zinc-alpha-2-glycoprotein [1] | 12.7 ± 1.5 | 14.3 ± 1.5 | 16.3 ± 1.5 |
| P07355 | Annexin A2 [23,44] | 12.7 ± 1.2 | 36.7 ± 3.5 | 25 ± 1 |
| P18510 | Interleukin 1 receptor antagonist protein [49] | 9.7 ± 1.5 | 12.3 ± 1.5 | 7 ± 1 |
| Q8WUM4 | Programmed cell death 6-interacting protein [50,51] | 9 ± 0 | 2.7 ± 0.6 | 5 ± 1.7 |
| P23528 | Cofilin-1 [1] | 8.7 ± 3.8 | 15.3 ± 2.1 | 8.7 ± 2.1 |
| P07476 | Involucrin [52] | 6 ± 1 | 3 ± 1.7 | 20.3 ± 2.3 |
| P01023 | Alpha-2-macroglobulin [22,52] | 5.3 ± 1.5 | 15.7 ± 2.3 | 7.7 ± 1.2 |
| Q6UXB2 | C-X-C motif chemokine ligand 17 [53] | 1 ± 1 | 0.7 ± 1.2 | 1 ± 1 |
| (2) Common to two batches | ||||
| P98088 | Mucin-5AC [44,54,55] | 10.3 ± 0.6 | 54.3 ± 3.8 | 0 |
| Q14116 | Interleukin 18 [56] | 0.7 ± 0.6 | 2 ± 1 | 0 |
| P02778 | C-X-C motif chemokine ligand 10 [57] | 2 ± 0 | 0 | 0.3 ± 0.6 |
| (3) Unique to one batch | ||||
| Q9UHD0 | Interleukin 19 [58] | 0.7 ± 1.2 | 0 | 0 |
| P14780 | Matrix metallopeptidase 9 * [49,59,60] | 0 | 0.3 ± 0.6 | 0 |
| P29508 | Serpin B3 [61] | 0 | 0 | 23.7 ± 2.5 |
| P48594 | Serpin B4 [61] | 0 | 0 | 12 ± 1 |
| Q9UHA7 | Interleukin 36 alpha [62] | 0 | 0 | 0.3 ± 0.6 |