Figure 5.

Detection limit and reproducibility of qPCR on Salmonella enteritidis Sdf1 and attB genes: (A) Sdf1 standard curve generated from quantification cycle numbers of a 10-fold dilution series of an amplicon product of 103 bp cloned into a pLB plasmid vector (Lethal Based Fast Cloning kit) plasmid vector in DNase- and RNase-free water (from 10⁰ to 10⁶). (B) Sdf1 standard curve generated from quantification cycle numbers of a 10-fold dilution series of an amplicon product of 199 bp cloned into a pLB plasmid vector (Lethal Based Fast Cloning kit) in DNase- and RNase-free water (from 10⁰ to 10⁶).