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. 2021 Dec 29;14(1):49. doi: 10.3390/v14010049

Figure 2.

Figure 2

Treatment with IFN inhibitors enhanced RABV production in MRC-5 cells.MRC-5 cells were infected with the PM RABV strain at an MOI of 0.05. Vero cells were infected with the aG RABV strain at an MOI of 0.001. The MRC-5 cells were treated with 4 μM TPCA-1 or ruxolitinib 2 h before RABV infection. (A) Total RNA from cells was extracted at 24 hpi, and the expression of IFN-α, IFN-β, STAT1, IRF7, and OAS1 was detected by qRT-PCR. RABV mRNA expression, antigen G concentration, and viral titer in culture supernatant were measured by qRT-PCR (B), enzyme-linked immune-sorbent assay (ELISA) (C), and focus-forming unit (FFU) assays (D), respectively, at 96, 168, and 240 hpi. +: RABV infection; * p < 0.05; ** p < 0.01, *** p < 0.001; **** p < 0.0001; ns p ≥ 0.05.