Table 3.
Antimicrobial activity in terms of surviving cells (%) of selected biocides at different concentrations (in µg/mL) against regrown biofilms of untreated and persister cells of B. cereus and P. fluorescens. Values are mean ± SDs of three independent assays with two replicates.
| B. cereus | P. fluorescens | ||||
|---|---|---|---|---|---|
| Untreated | Persister | Untreated | Persister | ||
| BAC | 10 | 12 ± 7 | 28 ± 10 | 72 ± 23 | 78 ± 25 |
| 100 | 4 ± 3 | 4 ± 3 | 28 ± 10 * | 77 ± 22 * | |
| GA | 1000 | 20 ± 5 | 37 ± 23 | 52 ± 33 | 64 ± 29 |
| 10,000 | 16 ± 4 | 34 ± 26 | 25 ± 21 | 40 ± 27 | |
| GO | 5000 | 0.5 ± 0.3 * | 6 ± 3 * | 44 ± 24 * | 2 ± 3 * |
| 20,000 | 0.6 ± 0.3 | 0.8 ± 0.3 | 0.5 ± 0.7 | 9 ± 9 | |
| PAA | 10 | 5 ± 4 | -- | 58 ± 30 | 35 ± 21 |
| 1000 | 0.2 ± 0.1 | -- | 2 ± 4 | 23 ± 24 | |
* Surviving cells (%) of regrown biofilms of persister cells were statistically different from regrown biofilms of untreated cells (unpaired t-test with Welch’s correction, p < 0.05).