Figure 7.

NMR-based metabolomics analysis of ileum. (A) O-PLS-DA plot and cross validation of ileum samples from young (blue) and old (orange) mice (left), with n = 5 each, and permutation between predictive component (p1) and three orthogonal components (o1, o2, o3) with cross-validated R²X, R²Y, and Q² coefficients (middle), and histogram showing permutation test with permutation number n = 100 (right). (B) Volcano plot of differentially regulated metabolites in ilea of young and aged mice. Increased (red) and decreased (blue) metabolites delimitate the fold change over aging, while grey dots denote insignificantly changed metabolites.