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. 2022 Jan 16;14(1):160. doi: 10.3390/v14010160

Figure 4.

Figure 4

Infectivity of HST and Z29 in HSB2-nec2δ and HSB2-CD134 cells. (A) Viral antigen detection in HSB2-nec2δ, HSB2-CD134, and HSB2-cont cells determined using an immunofluorescence assay. Cells were infected with HHV-6B HST and Z29 strains (multiplicity of infection = 0.1) and then treated with cold acetone at 48 h post-infection. HHV-6 p41 protein (red) was detected in virus-infected HSB2-nec2δ and HSB2-CD134 cells. HSB2-cont cells were used as a negative control. Nuclei of cells were stained with DAPI (blue). Arrowhead indicates balloon-like cells. Scale bars represent 50 μm. (B) Virus-infected cells (red) were counted. Error bars represent standard deviations (n = 3 per cell). Asterisks and ns indicate statistical significance (**** p < 0.0001, Tukey’s multiple comparison test) and no significance, respectively.