Effects of LFA1-P on the targeting of ICG-labeled GT DcNPs in 4T1 breast cancer lung metastasis models in mice. The mice inoculated with 4T1-luc breast cancer cells were administered with a single dose of ICG-labeled GT DcNP-LFA1-P (1%) and GT DcNP-LFA1-P (2%) (containing 50/5 mg/kg GT, IV). These mice were imagined for ICG fluorescence and presented as whole-body images (panel A), lung ICG intensity under whole-body image (panel B), and the ex vivo lung ICG intensity after removal of lung tissues (panel C). The ratio of the GT DcNP localization in GI and lungs was presented as the gastrointestinal (GI)/lung ICG intensity (panel D). The effects of LFA1-P density on the GT DcNP were determined with 0, 1%, and 2% of the total lipid excipient concentration of the ICG-GT DcNP, as described in each panel. The data are expressed as mean ± SD (n = 3). p-values were obtained from two-tailed t-tests.