Figure 3.
Regulatory role of BAY11-7082 and SB203580 in p38 and NF-κB activation in platelets. Washed platelets (1.2 × 109 cells/mL) were preincubated with a solvent control (0.1% DMSO), BAY11-7082 (6 μM), or SB203580 (20 μM), followed by collagen treatment (1 μg/mL) to trigger (A) p38, (B) IKK, and (C) p65 phosphorylation or (D) IκBα degradation for the immunoblotting study. Data are presented as the mean ± standard error of the mean (n = 4). ** p < 0.01, and *** p < 0.001, compared with the resting control (group treated with Tyrode’s solution); # p < 0.05, ## p < 0.01, and ### p < 0.001, compared with the 0.1% DMSO-treated group.