MEDICAL SCIENCES Correction for “Transcriptional suppression of AMPKα1 promotes breast cancer metastasis upon oncogene activation,” by Yong Yi, Deshi Chen, Juan Ao, Wenhua Zhang, Jianqiao Yi, Xiaokun Ren, Junjie Fei, Fengtian Li, Mengmeng Niu, Hu Chen, Yangkun Luo, Zhijun Luo, and Zhi-Xiong Jim Xiao, which was first published March 19, 2020; 10.1073/pnas.1914786117 (Proc. Natl. Acad. Sci. U.S.A. 117, 8013–8021).
The authors note that Fig. 1 appeared incorrectly. There were two errors in the published figure: Error #1: Inadvertent duplication of the images derived from transwell assays shown in Fig. 1H, Middle panel (shAα1–1) and Right panel (shAα1–2). Error #2: Unintentional error of using incorrect image derived from transwell assays in Fig. 1K, Right panel (Aα1-CA). The corrected figure and its legend appear below. The online version has been corrected.
Fig. 1.
Down-regulation of AMPKα1 expression is associated with advanced breast cancers and poor clinical outcomes. (A and B) Human breast cancer tissue microarrays either consisting of primary breast cancer samples (n = 22), breast cancer distant metastasis samples (n = 10), and nontumor breast samples (n = 13) (A) or consisting of breast cancer samples at different stages (grade 1, n = 46; grade 2, n = 61; grade 3, n = 32) (B) were subjected to IHC staining for AMPKα1 (Left, scale bar, 50 μm) with quantitative analyses using average optical density (AOD) (Right), as described in the Materials and Methods. (C and D) The Oncomine Bittner or Weigelt breast cancer dataset was used to analyze AMPKα1 mRNA levels in primary breast cancer samples and distant metastatic breast cancer samples (C) or in breast cancer samples at different stages (D). (E and F) RFS in patients with breast cancer was analyzed using AMPKα1 protein expression (AOD) values derived from B (E) or using AMPKα1 mRNA expression derived from Kaplan-Meier Plotter database (F). (G–L) Alteration of AMPKα1 expression affects breast cancer cell invasion in vitro and cancer metastasis in vivo. HCC1806 cells stably expressing shAMPKα1-1 (shAα1-1), shAMPKα1-2 (shAα1-2), or Hs578T cells stably expressing wild-type flag-AMPKα1 (Aα1-WT), constitutively active mutation flag-AMPKα1-T172D (Aα1-CA) were subjected to Western blot analyses (G and J) or transwell assays for cell invasion (H and K). (Scale bars, H and K, 50 μm.) For the in vivo tumor metastasis assays, 2 × 106 cells (HCC1806 or Hs578T) were i.v. injected into nude mice (n = 6/group). On day 55 (HCC1806) or day 45 (Hs578T), lungs were dissected, fixed, sectioned, and stained by hematoxylin and eosin (HE) for histological analyses. The numbers of metastatic nodules in the lungs per mouse were shown (I and L). (Scale bars, 0.5 mm.) Data are presented as means ± SEM. ***P < 0.001.