ATAC Sequencing Protocol For Cryopreserved Mammalian Cells

Abstract

ATAC-seq (assay for transposase-accessible chromatin with high-throughput sequencing) is a powerful method to evaluate chromatin accessibility and nucleosome positioning at a genome-wide scale. This assay uses a hyperactive Tn5 transposase, to simultaneously cut open chromatin and insert adapter sequences. After sequencing, the reads generated through this technique are generally indicative of transcriptional regulatory elements that are located in accessible chromatin. This method was originally developed by Buenrostro et al. (2013), and since then it has been improved by the same authors several times, until their last update called OMNI ATAC-seq ( Corces et al., 2017 ). Here, we describe an ATAC-seq protocol based on the OMNI-ATAC method, with a special focus on the initial steps of thawing cryopreserved cells, and the final steps of library purification using magnetic beads. This protocol can be of interest for laboratories working in a fast-paced environment.

Graphic abstract:

Flowchart of the protocol

Background

In eukaryotic organisms, the packaging of long DNA molecules into the nucleus is achieved by wrapping the DNA around histone proteins to form nucleosomes, and further compacting this structure into chromatin (Kornberg, 1974). The control of chromatin condensation is a major player in the regulation of gene expression. Since high DNA compaction restricts access for the transcriptional machinery, closed chromatin is usually associated with repressed gene expression. By contrast, open chromatin is associated with active gene expression, and the presence of regulatory elements like enhancers and promoters (Wegel and Shaw, 2005). The potential to reveal areas of high and low transcriptional activity has led to genome-wide mapping of chromatin accessibility becoming an area of broad interest.

Until now, several technologies for assessing the genomic profile of chromatin compaction have been developed. Some examples are micrococcal nuclease sensitivity (MNase-seq) (Zaret, 2005), formaldehyde-assisted isolation of regulatory elements (FAIRE-Seq) ( Giresi et al., 2007 ), and deoxyribonuclease I hypersensitivity (DNase-Seq) ( Boyle et al., 2008 ). In 2013, Greenleaf and colleagues developed a new method to probe chromatin accessibility, called assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq). ATAC-seq is a technique that assesses open chromatin using a mutated hyperactive Tn5 transposase, which simultaneously cuts DNA and tags the resulting fragments with sequencing adaptors, preferentially in decondensed chromatin areas ( Buenrostro et al., 2013 ). DNA sequencing libraries are then generated by PCR amplification and analyzed by high-throughput sequencing. The main advantages of ATAC-seq over the methods mentioned above are the low number of cells required as input and the length of the protocol (see Sun et al., 2019 for a full comparison between several chromatin accessibility assays).

Since development of the ATAC-seq method, the original authors have advanced ( Buenrostro et al., 2015 ) and improved (OMNI ATAC-seq, Corces et al., 2017 ) the protocol, to overcome some of the initial limitations of this technique. For example, to remove mitochondrial DNA from the transposition reaction, the OMNI ATAC-seq protocol includes a washing step with non-ionic detergent after the cell lysis. Another improvement is the addition of PBS in the transposition mix, to reduce the background noise. Other researchers from different laboratories have also helped to improve and optimize ATAC-seq protocols ( Fujiwara et al., 2019 ), or have adapted them for specific organisms and cell lines ( Doganli et al., 2017 , Shashikant and Ettensohn, 2019).

Being a core sequencing facility, our group handles a large number of ATAC-seq projects on a regular basis. We have adopted the OMNI ATAC-seq protocol for most of the ATAC-seq experiments, with two main modifications: (i) to handle a large number of projects, cell samples from researchers are sent cryopreserved, to be stored and processed at a convenient time. In this protocol, we include a section focused on the process of thawing cryopreserved cells, which is very important to preserve cell viability, and therefore to the success of an ATAC-seq experiment. (ii) After the purification of the final PCR reaction with the different silica column DNA purification kits available in the market, it is very common to find primer dimers and unwanted DNA fragments, due to over/under transposase digestion or an excess of PCR cycles. Thus, instead of using silica column-based methods, we purify the ATAC-seq libraries with magnetic beads. In addition to the removal of impurities, this allows us to size select the DNA fragments as needed for different samples. The use of magnetic beads for this step also enables the integration of automation platforms for processing a large number of libraries ( Hess et al., 2020 ).

In summary, we present here an ATAC-seq protocol adapted from Corces et al. (2017) that can be used by sequencing core facilities and other laboratories that routinely handle large quantities of samples.

Materials and Reagents

1. Solid Phase Reversible Immobilization (SPRI) select beads (Beckman Coulter, catalog number: B23318). Store at room temperature.

2. High Sensitivity DNA Chips and reagents (Agilent Technologies, catalog number: 5067-4626). Store reagents at 4°C and DNA Chips at room temperature.

3. Hanks Balanced Salt Solution (Sigma, catalog number: H6648-500ML). Store at room temperature.

4. Phosphate Buffered Saline (PBS) pH 7.4 (Gibco, Thermofisher, catalog number: 10010-023). Store at 4°C.

5. Iscove’s Modified Dulbecco’s Medium (IMDM) (Gibco, Thermofisher, catalog number: 12440-053). Store at 4°C.

6. Fetal Bovine Serum (FBS) (Gibco,Thermofisher, catalog number: 10082147). Store at -20°C.

7. 1 M Tris-HCl pH 7.4 (Teknova, catalog number: T5074). Store at room temperature.

8. 5 M NaCl (Teknova, catalog number: S5845). Store at room temperature.

9. 1 M MgCl₂ (Teknova, catalog number: M0308). Store at room temperature.

10. Digitonin (Promega, catalog number: G9441). Dilute the original 2% digitonin solution 1:1 in water. Make aliquots and store at -20°C.

11. Tween-20 (Sigma/Roche, catalog number: 11332465001). Store at 4°C.

12. NP40 (Sigma/Roche, catalog number: 11332473001). Store at 4°C.

13. Tagment DNA Enzyme (transposase) and 2× TD buffer (Illumina, catalog number: 20034197). Store at -20°C.

14. NEBNext 2× MasterMix (New England Biolabs, catalog number: M0541S). Store at -20°C.

15. Zymo DNA Clean & Concentrator^TM-5 Kit (Zymo Research, catalog number: D4014). Store at room temperature.

16. DNase (Worthington, catalog number: LS002007)

17. 20,000 Units/mL DNase stock solution (see Recipes)

18. ATAC-Resuspension buffer (ATAC-RSB) (see Recipes)

19. ATAC-RSB with detergents buffer (see Recipes)

20. Wash out lysis buffer (see Recipes)

21. Transposition mix (see Recipes)

Equipment

1. Pipettes (P1000, P200, P20, P10)

2. Cell counter (Nexcelom, model: Cellometer Auto 2000 Cell Viability Counter)

3. ThermoMixer (Eppendorf, model: C with heated lid)

4. Thermal Cycler (Bio-Rad, model: T100)

5. Bioanalyzer (Agilent Technologies, model: 2100)

Software

1. Agilent Technologies 2100 Bioanalyzer 2100 Expert (Agilent Technologies, https://www.agilent.com/en/product/automated-electrophoresis/bioanalyzer-systems/bioanalyzer-software/2100-expert-software-228259)

Procedure

Notes:

1. Prepare buffers, mixes, and stock solutions as described in the Recipes section, before starting the protocol.

2. We advise starting with at least 2 biological replicates. If this is not available, 2 technical replicates should be prepared from the same sample.

1. Preparation of cryopreserved cells for ATAC-seq (day 1)

   Notes:

   1. Cell viability is one of the most important parameters for a successful ATAC-seq experiment. To avoid cell death and lysis, the cells from cryopreserved vials should be carefully thawed and rapidly transferred to a conducive environment.

   2. The addition of a DNase digestion step in this section will digest the DNA from lysed cells that could otherwise interfere with the final data and interpretations.

   1. Warm the cell culture media (IMDM + 10% FBS) to 37°C.

   2. Add 4 mL of warm media to a 15 mL tube (one tube per sample).

   3. Remove cryopreserved cell vials from the -80°C freezer and place them in a bucket with ice.

   4. Place the cryopreserved cell vials at 37°C (no more than 3 vials at a time).

   5. When cells are half thawed (typically 1-2 min), add 1:1 volume of warm media to the vial.

   6. Pipet up and down several times, until the cells are completely thawed.

   7. Immediately transfer the cells into the 15 mL tube with warm media.

   8. Spin cells down at 500 × g for 5 min at room temperature.

   9. Discard the supernatant and resuspend the cell pellet in 1 mL of warm media.

   10. Transfer the resuspended cells to a microfuge tube.

   11. Add 10 μL of the aliquoted DNase stock solution (see Recipe 1) to the resuspended cells and incubate for 30 min at 37°C (final DNase concentration in each sample is 200 Units/mL).

   12. Spin cells down at 500 × g for 5 min at 4°C.

   13. Discard the supernatant and resuspend the cell pellet with 1 mL of ice-cooled PBS (first PBS wash).

   14. Spin cells down at 500 × g for 5 min at 4°C.

   15. Repeat Steps A13-A14.

   16. Resuspend the final pellet with 1 mL of ice-cooled PBS, count cells, and estimate viability using a cell counter. Using a dual-fluorescence (e.g., acridine orange and propidium iodide) imaging-based automated cell counter gives rapid and fairly accurate cell counts and viability estimates.

2. Cell lysis, transposition, and DNA extraction (day 1)

   Notes:

   1. To perform a successful ATAC-seq experiment, it is crucial to use the right cell-to-transposase ratio. Using too few cells will over-digest the chromatin, causing enrichment of smaller DNA fragments that may get preferentially sequenced, increasing the background noise in the data. On the other hand, an excess of cells will result in under-digestion, causing production of large DNA molecules that may be difficult to sequence.

   2. The authors of the OMNI-ATAC manuscripts (Buenrostro et al., 2015; Corces et al., 2017) have optimized the number of cells for this assay to 50,000. Thus, we have used this number for our routine experiments. If more or less cells need to be used, the cell-to-reagents ratio should be maintained, by adjusting the volumes of all reagents accordingly.

      1. Spin 50,000 viable cells down at 500 × g for 5 min at 4°C in an Eppendorf centrifuge.

      2. Remove the supernatant very carefully, without disturbing the pelleted cells. This process can be difficult since sometimes the pellets are almost invisible. To avoid accidentally aspirating the cells, we recommend first using a P1000 pipette until the last 150-200 μL, then a P200 pipette until the last 15-20 μL is left in the tube, and finally removing the remaining volume with a P20 pipette. If during this process some of the cells are accidentally disturbed, a second centrifugation at 500 × g, for 5 min at 4°C will be required.

      3. To lyse the cells, add 50 μL of the cold ATAC-RSB with detergents buffer previously prepared (see Recipes 2 and 3).

      4. Resuspend the cells with a P200 pipette up and down three times, and immediately incubate on ice for 3 min. If preparing more than one sample, ensure that all the samples have the same incubation time.

      5. Add 1 mL of the wash-out lysis buffer (see Recipe 4) and invert the tube three times to mix.

      6. Spin down the resulting nuclei solution at 500 × g for 10 min at 4°C in an Eppendorf centrifuge.

      7. Aspirate the supernatant very carefully, as described in Step B2.

      8. Resuspend the nuclei pellet in 50 μL of the transposition mix (see Recipe 5), and mix it by pipetting up and down six times.

      9. Using a thermomixer, incubate the samples at 37°C with agitation (1,000 RPM) for 30 min. In this step, the transposase will fragment and tag the accessible DNA with adaptors.

      10. Extract the DNA from the transposed nuclei using a Zymo DNA Clean and Concentrator-5 kit, according to the manufacturer’s instructions. Elute DNA in 21 μL of elution buffer. At this step, DNA can be stored at -20°C, until ready to prepare the library by PCR amplification.

3. Library preparation (day 2)

   Notes:

   1. During this part of the protocol, the DNA that was fragmented and tagged with adaptors in section B is PCR amplified using barcoded primers.

   2. To pool up to 24 libraries together, a list of 24 barcoded oligos (Ad2.1-Ad2.24) is in Table 1.

   3. In our experience, when starting with approximately 50,000 cells, the number of PCR cycles to have a successful ATAC-seq experiment without library over-amplification is between 7 and 9. In case of high cell viability (over 80%), we run the PCR for 7 cycles. In cases of low viability (less than 50%), we do 9. However, to avoid the risk of size and GC content PCR bias, the appropriate number of cycles can be determined by qPCR. Buenrostro et al. (2015) has a full explanation on how to calculate the precise number of PCR cycles.

      1. Prepare the following PCR reaction mix in a PCR tube.

         25 μL of 2× NEBNext Master Mix

         2.5 μL of 25 µM primer Ad1

         2.5 μL of 25 µM barcoded primer Ad2.1

         20 μL of transposed DNA sample

      2. When preparing different samples in the same pool for sequencing, each individual sample PCR reaction mix will have a different barcoded primer Ad2.

      3. Perform PCR using the following cycling conditions:

         1 = 72°C for 5 min

         2 = 98°C for 30 s

         3 = 98°C for 10 s

         4 = 63°C for 30 s

         5 = 72°C for 1 min

         6 = Go to step 3, six times

         7 = Hold at 4°C

4. Library purification (day 2)

   Note: After the PCR is completed, double-sided bead purification is necessary, to remove primer dimers and fragments over 1,000 bp.

   • 1Perform a double-sided SPRI bead purification.

     1. Add 0.5× volume (25 μL) of SPRI beads to the PCR samples (50 μL), and pipet up and down 10 times to mix thoroughly.

     2. Quickly spin the tubes down for 3 s and incubate for 10 min at room temperature.

     3. Place PCR tubes in a magnetic rack for 5 min, or until the supernatant is clear.

     4. Carefully transfer the supernatant to a new PCR tube.

     5. Add 1.3× original volume (65 μL) of SPRI beads, and pipet up and down 10 times to mix thoroughly.

     6. Quickly spin the tubes down for 3 s and incubate for 10 min at room temperature.

     7. Place PCR tubes in a magnetic rack for 5 min or until the supernatant is clear.

     8. Carefully remove the supernatant and discard it.

     9. Wash beads twice with 200 µL of 80% freshly made ethanol.

     10. Eliminate ethanol traces by spinning the tubes down for 3 s, placing them back in a magnetic rack, and aspirating the ethanol with a P10 pipette tip.

     11. Resuspend beads in 20 μL of nuclease-free water.

     12. Quickly spin the tubes down for 3 s and incubate for 1 min at room temperature.

     13. Place PCR tubes in a magnetic rack for 5 min or until the supernatant is clear.

     14. Transfer 19 μL of supernatant to a new tube.

     15. Check fragment size distribution profiles of the samples by running 0.5 μL on high sensitivity chip on a Bioanalyzer (Figure 1).

     16. Samples can be stored at -20°C until the next step.

   Note: Figure 1A shows a typical ATAC-seq bioanalyzer profile, after the first bead clean-up. Depending on the initial cell viability, cell type, experimental treatment, number of transposed nuclei, and number of PCR cycles, the nucleosomal banding pattern may have a different outcome.

   Notes:

   1. In our experience, cells with very low viability (less than 30%), usually do not show good amplification after 9 cycles of PCR. Though these libraries can be further amplified and sometimes a pattern resembling the nucleosomal banding pattern may appear, the risk of PCR bias is very high for such samples, and we recommend not to proceed with the sequencing of such samples.

   2. If after 9 PCR cycles, samples with good starting cell viability do not show amplification, we recommend a few extra amplification cycles (no more than 4).

   3. After the first library purification, most of the experiments will still have primer dimers and/or fragments over 1,000 bp (Figure 1A and 1C). Another issue could be the presence of a big low molecular weight peak (3 to 4 times taller than the other 2 peaks, Figure 1E). The presence of this peak could be due to over-digestion with the transposase or over-PCR amplification. This big peak can be problematic during the sequencing process, due to the fact that the DNA in this peak will be preferentially sequenced, because of its lower molecular weight compared to the other two peaks. In all three cases, the library should be submitted to a second bead purification: (i) Libraries with primer dimers and fragments over 1,000 bp (Figure 1A) will need the double-sided bead clean-up described in Step D1. On this occasion, the beads ratio in Step D1e should be 1×, and the incubation times in Steps D1b and D1f should only be 5 min (Figure 1B). (ii) If the library only has primer dimers (Figure 1C), we recommend doing a left-side clean-up with a 1× beads ratio (Step D2) (Figure 1D). 3) If in both situations the big low molecular size peak is also present (Figure 1E), the beads ratio in Steps D2b and D1e should be 0.95× (Figure 1F).

   • 2If needed, perform a left-sided SPRI bead purification.

     1. Add nuclease-free water to the sample from Step E1n up to 50 μL.

     2. Add 1× volume (50 μL) SPRI beads to the PCR samples, and pipet up and down 10 times to mix thoroughly.

     3. Quickly spin the tubes down for 3 s and incubate for 5 min at room temperature.

     4. Place PCR tubes in a magnetic rack for 5 min or until the supernatant is clear.

     5. Carefully remove supernatant and discard it.

     6. Wash beads twice with 200 μL of 80% freshly made ethanol.

     7. Eliminate ethanol traces by spinning the tubes down for 3 s, placing them back in a magnetic rack, and aspirating the ethanol with a P10 pipet tip.

     8. Resuspend beads in 15 μL of nuclease-free water.

     9. Quickly spin the tubes down for 3 s and incubate for 1 min at room temperature.

     10. Place PCR tubes in a magnetic rack for 5 min or until the supernatant is clear.

     11. Transfer 14 μL of supernatant to a new tube.

     12. Run 1 μL of the sample with high sensitivity chip Bioanalyzer (Figure 1D).

     13. Samples can be stored at -20°C.

Table 1. List of barcoded primers.

Ad1_noMX:	AATGATACGGCGACCACCGAGATCTACACTCGTCGGCAGCGTCAGATGTG
Ad2.1_TAAGGCGA	CAAGCAGAAGACGGCATACGAGATTCGCCTTAGTCTCGTGGGCTCGGAGATGT
Ad2.2_CGTACTAG	CAAGCAGAAGACGGCATACGAGATCTAGTACGGTCTCGTGGGCTCGGAGATGT
Ad2.3_AGGCAGAA	CAAGCAGAAGACGGCATACGAGATTTCTGCCTGTCTCGTGGGCTCGGAGATGT
Ad2.4_TCCTGAGC	CAAGCAGAAGACGGCATACGAGATGCTCAGGAGTCTCGTGGGCTCGGAGATGT
Ad2.5_GGACTCCT	CAAGCAGAAGACGGCATACGAGATAGGAGTCCGTCTCGTGGGCTCGGAGATGT
Ad2.6_TAGGCATG	CAAGCAGAAGACGGCATACGAGATCATGCCTAGTCTCGTGGGCTCGGAGATGT
Ad2.7_CTCTCTAC	CAAGCAGAAGACGGCATACGAGATGTAGAGAGGTCTCGTGGGCTCGGAGATGT
Ad2.8_CAGAGAGG	CAAGCAGAAGACGGCATACGAGATCCTCTCTGGTCTCGTGGGCTCGGAGATGT
Ad2.9_GCTACGCT	CAAGCAGAAGACGGCATACGAGATAGCGTAGCGTCTCGTGGGCTCGGAGATGT
Ad2.10_CGAGGCTG	CAAGCAGAAGACGGCATACGAGATCAGCCTCGGTCTCGTGGGCTCGGAGATGT
Ad2.11_AAGAGGCA	CAAGCAGAAGACGGCATACGAGATTGCCTCTTGTCTCGTGGGCTCGGAGATGT
Ad2.12_GTAGAGGA	CAAGCAGAAGACGGCATACGAGATTCCTCTACGTCTCGTGGGCTCGGAGATGT
Ad2.13_GTCGTGAT	CAAGCAGAAGACGGCATACGAGATATCACGACGTCTCGTGGGCTCGGAGATGT
Ad2.14_ACCACTGT	CAAGCAGAAGACGGCATACGAGATACAGTGGTGTCTCGTGGGCTCGGAGATGT
Ad2.15_TGGATCTG	CAAGCAGAAGACGGCATACGAGATCAGATCCAGTCTCGTGGGCTCGGAGATGT
Ad2.16_CCGTTTGT	CAAGCAGAAGACGGCATACGAGATACAAACGGGTCTCGTGGGCTCGGAGATGT
Ad2.17_TGCTGGGT	CAAGCAGAAGACGGCATACGAGATACCCAGCAGTCTCGTGGGCTCGGAGATGT
Ad2.18_GAGGGGTT	CAAGCAGAAGACGGCATACGAGATAACCCCTCGTCTCGTGGGCTCGGAGATGT
Ad2.19_AGGTTGGG	CAAGCAGAAGACGGCATACGAGATCCCAACCTGTCTCGTGGGCTCGGAGATGT
Ad2.20_GTGTGGTG	CAAGCAGAAGACGGCATACGAGATCACCACACGTCTCGTGGGCTCGGAGATGT
Ad2.21_TGGGTTTC	CAAGCAGAAGACGGCATACGAGATGAAACCCAGTCTCGTGGGCTCGGAGATGT
Ad2.22_TGGTCACA	CAAGCAGAAGACGGCATACGAGATTGTGACCAGTCTCGTGGGCTCGGAGATGT
Ad2.23_TTGACCCT	CAAGCAGAAGACGGCATACGAGATAGGGTCAAGTCTCGTGGGCTCGGAGATGT
Ad2.24_CCACTCCT	CAAGCAGAAGACGGCATACGAGATAGGAGTGGGTCTCGTGGGCTCGGAGATGT

Figure 1. High Sensitivity Bioanalyzer profile of several ATAC-seq libraries after a variety of magnetic bead purifications.

A. ATAC-seq profile after the first double size selection clean-up. Primer dimers and fragments over 1,000 bp are still present in this library. B. To remove all the unwanted DNA, a second double size selection purification was performed. The peaks in the final library indicate the mono-, di-, and tri-nucleosome-associated fragments. C. ATAC-seq profile after the first double size selection clean-up, showing the presence of primer dimers. D. To remove all the primer dimers, a second clean-up as described in Step D2 was conducted. E. ATAC-seq profile after the first double size selection clean-up, showing the presence of primer dimers, fragments over 1000 bp, and a big first peak. F. To reduce the size of the low molecular weight peak, a 0.95× instead of a 1× beads ratio was applied during the second purification (see notes in section D for more details).

Data analysis

1. The minimum library quantity required for pooling and sequencing for any Illumina platform is 5 nM in 10 μL.

2. Add libraries in the same pool by equimolarity. We advise qPCR for accurate estimation of library concentrations.

3. We recommend paired-end sequencing on an Illumina platform NextSeq1000/2000 or NovaSeq6000, with 100 million minimum reads per sample with 2×100 bps read length, or NextSeq500/550, with 2×75 bps read length.

4. For downstream data analysis it is necessary to have at least two replicates per sample.

Recipes

1. 20,000 Units/mL DNase stock solution

   Make a 20,000 Units/mL DNase stock solution. Resuspend the lyophilized DNase powder (100 mg) in 10 mL of Hanks balanced salt solution (HBSS). This corresponds to 10 mg/mL. As 1 mg of DNase equals to 2,000 Units, this means that 10 mg/mL are 20,000 Units/mL. Aliquot the stock solution in microfuge tubes and keep them at 4°C for up to 3 weeks. Otherwise, keep the aliquots at -20°C.

2. ATAC-RSB buffer (Table 2)

   Prepare buffer ATAC-RSB as described in Table 2. This buffer can be stored at room temperature for months.

3. ATAC-RSB with detergents buffer (Table 3)

   Prepare ATAC-RSB with detergents buffer on the day of the experiment and store it at 4°C.

4. Wash-out buffer (Table 4)

   Prepare wash-out buffer on the day of the experiment and store it at 4°C.

5. Transposition mix (Table 5)

   Prepare transposition mix during step 6 in the ATAC-seq protocol section according to Table 5, and keep it on ice. If preparing more than one sample, add an additional excess of each reagent.

Table 2. Recipe for ATAC-RSB buffer.

Reagent	Initial concentration	Final concentration	Volume for 100 mL
Tris-HCl pH 7.4	1 M	10 mM	1,000 μL
NaCl	5 M	10 mM	200 μL
MgCl2	1 M	3 mM	300 μL
Nuclease-free water	-	-	98.5 μL

Table 3. Recipe for ATAC-RSB with detergents buffer (50 μL per sample).

Reagent	Initial concentration	Final concentration	Volume for 1,000 μL
NP40	10%	0.1%	10 μL
Tween-20	10%	0.1%	10 μL
Digitonin	1%	0.01%	10 μL
ATAC-RSB buffer	-	-	970 μL

Table 4. Recipe for wash out lysis buffer (1 mL per sample).

Reagent	Initial concentration	Final concentration	Volume for 10 mL
NP40	10%	0.1%	100 μL
ATAC-RSB buffer	-	-	9,900 μL

Table 5. Recipe for transposition mix (50 μL per sample).

Reagent	Initial concentration	Final concentration	Volume for 50 μL
TD buffer	2×	1×	25 μL
Transposase PBS Digitonin Tween-20 Nuclease-free water	20,000 nM - 1% 10% -	100 nM - 0.01% 0.1% -	2.5 μL 16.5 μL 0.5 μL 0.5 μL 5 μL

Competing interests

No competing interests to declare.