Figure 2.

CARS images of LDs in live MII oocytes matured in high-FA environments. Immature GV stage oocytes were incubated overnight in standard M2 medium (A, E, I) supplemented with 200 µM PA (B, F, J), 200 µM OA (C, G, K) or a combination of 200 µM PA and 200 µM OA (D, H, L). MII oocytes were then selected for DIC (A, B, C, D) and CARS imaging (E, F, G, H). CARS images are colour-coded; colour bar shows depth colour-coding from −25 to 25 μm of 101 z-stacks (0 μm being the approximately equatorial plane of the egg or embryo). (I, J, K, L) are representative histograms of the number of LDs making up clusters for the different growth conditions above.