Figure 5.

Intracellular Ca²⁺ response of living MII oocytes matured in high-FA environments. (A) After IVM, MII oocytes were injected with a mixture of OGBD and Alexa594 (in 10:1 ratio) for ratio-metric fluorescent measurements of intracellular Ca²⁺ store. The ER Ca²⁺ store was measured by the thapsigargin (10 μM)-induced Ca²⁺ response, and all other intracellular Ca²⁺ store was measured by the ionomycin (5 μM)-induced Ca²⁺ response. (B) The experiment in (A) was carried out on MII oocytes matured with various FA condition. The average fluorescence ratio of the resting state (red bar), thapsigargin response (blue bar) and Ionomycin response (yellow bar) was calculated form multiple oocytes (n = 55, 27 or 24 for control, PA or OA condition, respectively), using two or three mice trials. For the resting state, both PA and OA condition showed no significant difference (P  > 0.1) from controls. There were also no differences for the thapsigargin response (P > 0.5) and the Ionomycin response (P  > 0.1) from controls.