Figure 6.

CARS, TPF and DIC images in living MII oocytes matured in different environments. For IVM, immature GV stage oocytes were incubated overnight in standard M2 medium (A, B, C, D) or supplemented with 200 µM PA (E, F, G, H), 200 µM OA (I, J, K, L) or combination of 200 µM PA and 200 µM OA (P, Q, R, S). MII oocytes were then selected and injected with neuro-DiO in a soybean oil droplet. (A, E, I and M) DIC, (C, G, K and O) false-coloured CARS images at wavenumber 2850 cm⁻¹ and (B, F, J and N) TPF xy images accompanied by (D, H, L and P) false-coloured overlays scale bars: 10 μm. The bright circles inside the oocytes are due to the oil drop that was injected to load neuro-DiO into the ER.