Figure 5. Effect of acquisition time and number of accumulations on Raman spectrum of bone.

Raman spectra were acquired from the native unpolished human cortical bone obtained from a cadaveric femur using an 830 nm laser source while keeping the total acquisition time constant (e.g., scan time × number of accumulations = 60 s) but different combinations of scan time × number of accumulations. The spectral resolution of the Raman instrument was 1 cm⁻¹.