Oxidative ornithine metabolism supports non-inflammatory C. difficile colonization

Abstract

The enteric pathogen Clostridioides difficile (Cd) is responsible for a toxin-mediated infection that causes more than 200,000 recorded hospitalizations and 13,000 deaths in the United States every year¹. However, Cd can colonize the gut in the absence of disease symptoms. Prevalence of asymptomatic colonization by toxigenic Cd in healthy populations is high; asymptomatic carriers are at increased risk of infection compared to noncolonized individuals and may be a reservoir for transmission of Cd infection^2,3. Elucidating the molecular mechanisms by which Cd persists in the absence of disease is necessary for understanding pathogenesis and developing refined therapeutic strategies. Here, we show with gut microbiome metatranscriptomic analysis that mice recalcitrant to Cd infection and inflammation exhibit increased community-wide expression of arginine and ornithine metabolic pathways. To query Cd metabolism specifically, we leverage RNA sequencing in gnotobiotic mice infected with two wild-type strains (630 and R20291) and isogenic toxin-deficient mutants of these strains to differentiate inflammation-dependent versus -independent transcriptional states. A single operon encoding oxidative ornithine degradation is consistently upregulated across non-toxigenic Cd strains. Combining untargeted and targeted metabolomics with bacterial and host genetics, we demonstrate that both diet- and host-derived sources of ornithine provide a competitive advantage to Cd, suggesting a mechanism for Cd persistence within a non-inflammatory, healthy gut.

Using a combination of metabolomics and bacterial and host genetics, Pruss et al. show that upregulated oxidative ornithine metabolism in Clostridioides difficile promotes its persistence within the gastrointestinal tract under non-inflammatory conditions.

Main

Clostridioides difficile (recently reclassified from Clostridium, here referred to as Cd) is the leading cause of nosocomial diarrhea worldwide. Cd causes disease by the secretion of large glycosylating toxin proteins⁴; in susceptible hosts, infection can result in toxic megacolon or death. An important facet of Cd biology is its persistence in humans in the absence of symptoms, termed asymptomatic carriage. Prevalence reports vary widely^2,5–9; up to 71% of infants and 15% of healthy adults may asymptomatically carry toxigenic Cd strains, with estimates increasing in populations with underlying conditions². Mechanisms underlying this aspect of Cd lifestyle remain obscure; an improved understanding of Cd metabolic behavior in non-inflammatory (homeostatic) versus inflammatory (generated by toxin production) conditions will better inform addressing and preventing disease progression.

Detailed investigations of Cd during asymptomatic carriage are challenging due to difficulties associated with (1) procuring sufficient human samples in the absence of diarrhea or disease, (2) the relatively low abundance of Cd in the human microbiota and (3) experimentally controlling toxin production in vivo. An approach we¹⁰ and others¹¹ have taken to understand how toxin production in vivo alters Cd metabolism utilizes isogenic mutant strains of Cd with impaired toxin production; however, these studies focused on wild-type (WT) infection and attention toward Cd persistence in the absence of disease is warranted.

A previous study showed that humans colonized with naturally occurring non-toxigenic Cd isolates (n = 4) had distinct fecal metabolomes from those infected with toxigenic strains (n = 6)¹², suggesting that microbial community metabolism was influenced by disease symptoms imparted by toxin production. To further understand changes to community metabolism during inflammatory versus non-inflammatory Cd infection (CDI), we examined metatranscriptomic data from a clinically relevant mouse model. Patients with diarrhea provided stool samples for microbiota sequencing; samples were classified as healthy-like or dysbiotic based on symptoms and microbial composition (Fig. 1a). Mice humanized with stool samples from healthy-like patients were recalcitrant to CDI, whereas mice humanized with feces from dysbiotic donors sustained high levels of Cd and higher inflammation scores¹³. Here, we compared expression of community-level pathways in cecal metatranscriptomes from these mice.

Fig. 1. Community amino acid metabolic pathways differ in CDI-susceptible hosts.

a, Experimental design. Samples from patients with diarrhea were collected, classified as healthy-like or dysbiotic and used for a humanized mouse model of CDI¹³. Mice humanized with healthy-like microbiotas are recalcitrant to developing CDI and associated pathology, whereas mice colonized with communities from dysbiotic, symptomatic patients harbor persistent infections and higher levels of inflammation. RNA-seq was performed on n = 6 mice per group. b, Community-level amino acid metabolic pathways enriched in mice humanized with healthy-like human microbiotas (positive log₂fold change and log₁₀adjusted P value) versus dysbiotic (negative). Metabolic pathways involved in ornithine, arginine and polyamine metabolism are colored in red. c, Differential expression of arginine, ornithine and polyamine metabolic pathways after Cd challenge.

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Hundreds of pathways were differentially expressed between dysbiotic (82 pathways enriched) and healthy-like (219 pathways) humanized mice. Of these, 28 pathways involved in amino acid metabolism were more highly expressed in healthy-like mice (13% of total differentially expressed), whereas only 6 amino acid metabolic pathways were enriched in dysbiotic mice (7%; Fig. 1b). The increase in expression of pathways involved in amino acid metabolism in healthy-like, Cd-resistant communities was accompanied by reduced levels of several amino acids in feces¹³. Several pathways involved in arginine, ornithine and polyamine metabolism were more highly expressed in healthy communities (Fig. 1b). These inter-related metabolic pathways were of interest as the metabolism of arginine and ornithine into polyamines enhances gut barrier function by increasing expression of tight junction proteins and lessening inflammatory responses in immune cells^14–16. In a separate study that conducted metabolomic profiling of humans infected with Cd versus healthy controls, microbial amino acid metabolites were the strongest differentiators of Cd colonization; of these, ornithine had the second-highest odds ratio¹⁷. Furthermore, excess arginine and arginine di-peptides increase toxin production in some Cd strains in vitro¹⁸. Of the 11 arginine/ornithine/polyamine pathways enriched in healthy-like microbiotas before CDI, 6 remained more highly expressed after exposure to Cd (Fig. 1c). In dysbiotic mice, no pathways involved in arginine, ornithine nor polyamine metabolism were enriched after CDI (Fig. 1c). These results suggest the potential for community amino acid metabolism in enhancing host barrier function and playing a role in maintaining the gut in a non-inflammatory state when challenged with Cd. However, the role of Cd amino acid metabolism within recalcitrant versus susceptible communities remains a difficult question to address in the context of a complex microbiota.

To query changes to metabolism due specifically to Cd-induced inflammation versus the presence of Cd in a commensal (non-inflammatory) state, we employed toxin-possessing (WT) and toxin-deficient (Tox⁻) isogenic mutants of two Cd strains in defined communities of commensal microbes in gnotobiotic mice. Germ-free mice were colonized with a three-member community: Bacteroides thetaiotaomicron, Escherichia coli and Clostridium sporogenes (Defined Community 1, DC1), members of the three most abundant phyla in the adult human gut microbiota. After 12–15 d of colonization, mice were infected with either WT Cd (630 WT) or the toxin-deficient isogenic mutant (630 TcdA⁻B⁻, here referred to as 630 Tox⁻, which has insertional mutations in each of its genes encoding toxin proteins, tcdA and tcdB¹⁹; Extended Data Fig. 1a). WT 630 induced significantly more pathology than Tox⁻-colonized mice, which did not differ significantly from uninfected controls (Fig. 2a). We were interested in comparing Cd 630 with a hypervirulent strain, R20291, which produces a third toxin (binary toxin, CDT), increased levels of TcdA and TcdB²⁰ and has been associated with more recent hospital outbreaks²¹. WT R20291 induced greater overall tissue damage (Fig. 2a) and more inflammatory cell infiltrates than 630 WT (Fig. 2b). Colonization with the isogenic triple-toxin knockout, which contains mutations in all three toxin genes (tcdA, tcdB, cdt; TcdA⁻B⁻CDT⁻ (ref. ²²), here referred to as R20291 Tox⁻), led to an identical histopathology score as colonization with 630 Tox⁻ and both uninfected control groups (Fig. 2a). WT Cd reached a higher relative abundance than its toxin-deficient counterpart (Extended Data Fig. 1b), indicating that toxin production confers a competitive advantage to Cd in vivo.

Extended Data Fig. 1. Cd toxin production confers advantage in vivo and generates distinct metabolome.

a, Experimental gnotobiotic mouse model for Cd colonization in non-inflamed conditions. Germ-free mice were tri-colonized with B. thetaiotaomicron, C. sporogenes and E. coli 12–15 days prior to infection with WT (630 or R20291) or Tox- (630 TcdA⁻TcdB⁻ or R20291 TcdA⁻TcdB⁻CDT⁻ Cd or gavaged with PBS. Mice were maintained on standard diet and no antibiotic pretreatment was used. b, WT R20291 reaches a higher relative abundance in the presence of a defined commensal community (DC 1, mean ± s.e.m., n = 5 mice/group, unpaired two-tailed t-test) than its toxin-deficient counterpart. Absolute abundance (CFU/mL feces) of the two strains did not differ (data not shown). c, R20291 toxin production differentiates the metabolic milieu of cecal contents. Principal components analysis with 95% confidence regions for mice infected with WT (n = 5 mice, red), Tox- (n = 5, purple) or uninfected controls (n = 4, yellow).

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Fig. 2. Putative l-ornithine oxidative degradation locus consistently differentially expressed in gnotobiotic models for Cd metabolism in a non-inflamed environment.

a, Hypervirulent WT Cd R20291 elicits a significantly greater magnitude of pathology in the cecal blind tip and proximal colon of mice than all other groups, including WT Cd 630. Both WT infections (630 and R20291) incite significantly higher pathology than all Tox⁻ and uninfected control (crtl) groups, which do not differ from one another (one-way analysis of variance (ANOVA) F_(5,22) = 180.3 with Tukey’s post hoc comparisons. WT 630 versus R20291 Tox⁻ and control group, P < 0.0001; WT R20291 versus 630 Tox⁻ and control group, P < 0.0001; n = 5 mice per group except for the 630 uninfected control where n = 4; bars denote mean ± s.e.m.). b, Histopathological score breakdown for mice infected with WT R20291 or WT 630. R20291 leads to a significantly higher level of inflammatory cell infiltration (multiple unpaired Student’s t-tests with Benjamini, Krieger, Yekutieli two-stage linear step-up procedure; n = 5 mice per group; bars denote mean ± s.e.m.). c, Extent of differential gene expression between WT and Tox⁻ Cd across different gnotobiotic settings (DESeq2 Wald test). DC1 (n = 5 WT, n = 4 Tox⁻); DC2 (630, n = 4 mice per group; R20291, n = 5 mice per group). d, Combined differential gene expression between 630 WT (positive fold change) and 630 Tox⁻ (negative) combined across both defined communities (adjusted P value < 0.01, n = 9 WT, n = 8 Tox⁻). e, Normalized expression values (reads per kilobase million) of a putative l-ornithine degradation locus, the only genomic locus consistently differentially expressed across Cd strains and two different defined communities (box-plots denote interquartile range, whiskers denote min to max). f, Schematic of the ornithine oxidative degradation pathway; l-ornithine undergoes racemization to d-ornithine (orr, ornithine racemase), migration of the amino group from C5 to C4 (oraSoraE, d-ornithine aminomutase), a dehydrogenase reaction generating ammonia (ord, 2,4-diaminopentanoate dehydrogenase), CoA-mediated thiolytic cleavage generating acetyl-CoA (ortAortB, 2-amino-4-ketopentanoate thiolase) leading to ATP production via acetyl phosphate.

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We performed untargeted metabolomics on cecal contents of mice infected with R20291 WT, R20291 Tox⁻ or uninfected controls. Identified metabolites of the gut lumen differentiate mice infected with toxin-producing Cd from Tox⁻ or the uninfected group (Extended Data Fig. 1c). Several amino acids and their bacterial metabolites were altered across infection conditions (Extended Data Fig. 2a and Supplementary Table 1). Notably, ornithine, putrescine (one product of microbial ornithine metabolism) and citrulline (a potential precursor) differed significantly across groups (Extended Data Fig. 2b). 5-Aminovalerate, the reductive product of ornithine and/or proline^17,23,24, was higher in the absence of toxin-induced inflammation (Extended Data Fig. 2b). These metabolomics data combined with the community-level transcriptional pathway analysis in susceptible versus resistant humanized mice support the notion that metabolic pathways involving arginine, ornithine and putrescine are more active in the absence of Cd-induced inflammation.

Extended Data Fig. 2. Untargeted metabolomics demonstrates toxin-dependent changes to metabolites in gut lumen.

a, 75 metabolites with the greatest variance across groups of mice infected with R20291 WT (n = 5 mice), Tox- (n = 5) or uninfected controls (n = 4) as described in Extended Data Fig. 1a (row-normalized log_10-transformed peak areas shown). Amino acids are noted with asterisks; metabolites in (b) are highlighted in red. b, Normalized peak values for identified metabolites related to ornithine metabolism. Ornithine and its reductive end-product 5-aminovaleric acid exhibit inverse trends (group sizes are as in a, mean ± s.e.m. shown; one-way ANOVA across all named metabolites with FDR-adjusted P value (Padj) < 0.1, * denotes significance by Fisher’s LSD post hoc comparison: ornithine F = 5.5103, Padj = 0.0849; citrulline F = 20.078, Padj = 0.0029, 5-aminovaleric acid F = 5.3369, Padj = 0.0895; putrescine F = 11.315, Padj = 0.0162).

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To examine the metabolic behavior of Cd itself, we isolated RNA from cecal contents of mice 5 d after infection with WT (630 or R20291), Tox⁻ (630 or R20291) or uninfected controls for RNA-seq (Extended Data Fig. 1a). Using a defined commensal community allowed for sufficient coverage of the Cd transcriptional profile for differential expression analysis, which was not possible in a complex, humanized microbiota¹³. R20291, which elicited a greater degree of tissue damage (Fig. 2a) and increased inflammatory cell infiltrates (Fig. 2b), exhibited a far greater extent of differential gene expression due to toxin production than 630 (Fig. 2c and Supplementary Tables 2 and 3). We employed a second defined community (Defined Community 2, DC2), consisting of Edwardsiella tarda, Clostridium scindens and Bacteroides thetaiotaomicron, to determine whether the limited transcriptional response of Cd 630 was specific to the commensals of DC1. Again, few genes were differentially expressed by 630 in the presence or absence of toxin production (Fig. 2c). We combined Cd 630 gene expression across both defined communities to determine which genes were differentially expressed due to toxin production independent of the specific community members present (Fig. 2d and Supplementary Table 2). A gene annotated as a glycerol kinase (glpK1) was most highly enriched in WT, as well as two neighboring genes putatively annotated as a pyridoxal phosphate-dependent aminotransferase (CD630_07400) and an ethanolamine utilization protein (CD630_07420). WT R20291 also upregulated ethanolamine utilization (Supplementary Table 3), consistent with enteric pathogen ethanolamine metabolism in inflammatory conditions^25–27.

In the absence of inflammation (Tox⁻), pathways for butyrate fermentation were more highly expressed in both hypervirulent R20291 (Supplementary Table 3) and 630 (DC2; Supplementary Table 2). Despite the smaller magnitude of differential expression in 630 compared to R20291 (Fig. 2c), a single transcriptional unit was differentially expressed across both commensal communities and was shared with R20291. A putative oxidative l-ornithine degradation pathway was more highly expressed by 630 Tox⁻ and R20291 Tox⁻ (Fig. 2e), suggesting that this pathway supports Cd metabolism in a non-inflamed gut.

Ornithine metabolism has not been investigated in Cd previously; based on homology in other members of the genus Clostridium, it is predicted that Stickland metabolism-based oxidative degradation of l-ornithine yields l-alanine, with the production of ATP via acetyl-CoA and ammonia (Fig. 2f)^28–31. While the Stickland reaction typically occurs in amino acid pairs, other Clostridia can utilize ornithine as both an electron donor and acceptor. In addition to the oxidative catabolism of l-ornithine (Fig. 2f), Cd encodes a reductive pathway that generates 5-aminovalerate through proline (Extended Data Fig. 3a). We and others have demonstrated a role for proline reduction in Cd persistence in vivo^13,24.

Extended Data Fig. 3. Cd ornithine degradation and biosynthesis pathways.

a, Annotated biosynthetic and degradative pathways of L-ornithine in Cd. b, R20291 WT exhibits increased growth yield in rich media (0.5X BHIS) supplemented with different concentrations of L-ornithine (mean ± s.e.m., n = 5/group; one-way ANOVA F_(2,12)=16.80 with Tukey’s post hoc comparisons). c, 630 WT exhibits increased growth yield in fully defined amino acid basal defined media (BDM) when supplemented with 8 mM L-ornithine (mean ± s.e.m., n = 5/group, unpaired two-tailed t-test). d, In vitro competition between 630 WT and mutants in L-ornithine degradation (∆oraSE n = 4) or biosynthesis (∆argF, n = 4; ∆argM, n = 3). Strains were co-cultured for 24 hours in amino acid minimal media (CDDM) supplemented with additional 50 mM L-ornithine (mean ± s.e.m.; ANOVA with post hoc comparisons: F_(2,7)=25.63,). A competitive index of 1 indicates equal abundance. e, In vitro competitive index between WT Cd and ∆oraSE in BDM supplemented with 8 mM L-ornithine or 8 mM L-arginine (mean ± s.e.m., n = 4/group; ANOVA with Dunnett’s post hoc comparisons using base medium as the control group: F_(2,9)=3.553). Strains were co-cultured for 48 hours. For all panels, * P < 0.05, ** P < 0.01, *** P < 0.001. f, WT Cd (n = 6) and ∆oraSE (n = 7) grow equally well in rich media (0.5X BHIS). Supplementation with 0.5% or 0.25% (w/v) L-ornithine leads to increased growth yield in WT but not the ornithine aminomutase mutant (n = 7/group, mean ± s.e.m.). g, In gnotobiotic mice harboring a defined community of bacteria, the ∆oraSE mutant exhibits increased expression of genes in the oxidative ornithine locus (middle panel) but not the reductive locus (right) nor biosynthesis of arginine (argF) or glutamine (argM). For f and g, multiple unpaired t-tests with Benjamini, Krieger and Yekutieli two-stage step-up FDR correction.

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Addition of l-ornithine to medium increased growth yield of both Cd R20291 and 630 (Extended Data Fig. 3b,c). We constructed deletion mutants in ornithine oxidative degradation (∆oraSE) and two separate predicted ornithine biosynthetic pathways from arginine/citrulline (∆argF) or glutamine/glutamate (∆argM; Extended Data Fig. 3a). ∆oraSE and ∆argF suffered a competitive disadvantage after 24 h compared to WT Cd in amino acid minimal medium, but ∆argM, involved in interconversion of glutamine and ornithine, does not (Extended Data Fig. 3d). These data suggest that ornithine oxidative degradation and biosynthesis from arginine/citrulline are more important than biosynthesis from glutamine for increasing culture density during growth on amino acids. Addition of ornithine increased the advantage of WT over ∆oraSE, whereas arginine did not, indicating a potential fitness advantage results from ornithine taken up from the environment, rather than derived from Cd’s own metabolism (Extended Data Fig. 3e). In rich medium, WT and ∆oraSE reached the same maximum optical density; addition of l-ornithine to rich medium increased growth of WT but not ∆oraSE (Extended Data Fig. 3f). To determine whether the ornithine aminomutase deletion affected other ornithine metabolic pathways in vivo, we infected gnotobiotic mice harboring a defined consortium of bacteria with either WT or ∆oraSE and isolated total RNA from cecal contents (Extended Data Fig. 3g). The mutant upregulated genes in the ornithine oxidative degradation locus (orr, ord and ortB), despite its inability to perform this metabolism. The ornithine reductive pathway (cyclodeaminase, prdF and prdD) as well as genes involved in ornithine interconversion with citrulline/arginine (argF) or glutamate/glutamine (argM) were unaffected.

To determine whether dietary ornithine availability influenced Cd metabolism, we compared transcriptional profiling³² of mice monocolonized with WT Cd fed standard rodent diet (which contains ornithine) or a fully defined diet lacking ornithine. Genes in the oxidative ornithine degradation locus were some of the most differentially expressed between dietary conditions: when dietary ornithine was present, genes involved in its oxidative metabolism were more highly expressed (Fig. 3a). To determine whether ornithine degradation conferred a fitness advantage in vivo, conventional microbiota-colonized mice were co-infected with equal amounts of WT and ∆oraSE Cd. WT outcompeted the ∆oraSE mutant in conventional mice fed standard diet containing ornithine, but not a fully defined diet devoid of ornithine (Fig. 3b). To test whether ornithine itself provided the competitive advantage to WT Cd over ∆oraSE, conventional mice were fed a fully defined diet devoid of ornithine and co-infected with WT and ∆oraSE. One group was administered 1% (w/v) l-ornithine in drinking water, which provided a competitive advantage to WT Cd over ∆oraSE; WT did not outcompete the mutant in mice when dietary ornithine was lacking (Fig. 3c).

Fig. 3. Metabolism of ornithine from diet confers competitive advantage to Cd and leads to reduced host inflammation.

a, The ornithine oxidative degradation pathway is significantly enriched in mice monocolonized with WT Cd on standard mouse chow compared to mice monocolonized with WT Cd provided a fully defined diet lacking ornithine (row-normalized z score for microarray data from ref. ³²; n = 4 mice per group). b, WT Cd has a competitive advantage in conventional mice over ∆oraSE strain in standard diet background, but not in a fully defined diet devoid of ornithine (unpaired two-tailed Student’s t-test, n = 3 mice per group; mean ± s.e.m.). c, 1% ornithine supplementation (w/v) to conventional mice on a fully defined ornithine-free diet provides a competitive advantage to WT Cd (pairwise Student’s t-tests; mean ± s.e.m., n = 5 mice per group). d, WT Cd achieves a higher absolute abundance than ∆oraSE in cecal contents of gnotobiotic mice fed an ornithine-free diet supplemented with ornithine in drinking water. c.f.u., colony-forming unit. e,f, ∆oraSE infection leads to higher levels of lipocalin-2 in serum in gnotobiotic mice harboring a defined consortium of bacteria (e) or conventional mice fed standard diet (f). For d–f data were analyzed by unpaired two-tailed Student’s t-tests; mean ± s.e.m., n = 5 mice per group.

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To determine whether dietary ornithine conferred a benefit in colonization levels, we infected gnotobiotic mice harboring a defined community with either WT or ∆oraSE. WT achieved significantly higher abundance in cecal contents of mice fed an ornithine-free diet supplemented with ornithine in drinking water (Fig. 3d). In mice with a complex microbiota (conventional) fed an ornithine-free diet, ornithine supplementation in drinking water led to higher overall Cd abundance in cecal contents (Extended Data Fig. 4a). Finally, WT achieved higher absolute abundance in feces (Extended Data Fig. 4b) and cecal contents (Extended Data Fig. 4c) when ornithine was supplemented in drinking water to standard diet compared to standard diet alone.

Extended Data Fig. 4. Dietary ornithine boosts Cd abundance in the mouse gut.

a, Ornithine supplementation (1% w/v in drinking water) to conventional mice fed an ornithine-free diet co-infected with equal amounts WT and ∆oraSE leads to higher overall Cd abundance in cecal contents (mean ± s.e.m., n = 5 mice/group; unpaired two-tailed t-test). WT Cd reaches higher abundance in feces (b, n = 5 mice/group, mean ± s.e.m.) and cecal contents (c, n = 5 mice/group, mean ± s.e.m.; unpaired two-tailed t-test) of conventional mice fed standard diet supplemented with 1% ornithine in drinking water. d, Dietary ornithine availability does not alter toxin production on a per-cell basis in mice harboring a defined consortium of bacteria infected with WT or ∆oraSE Cd fed an ornithine-free diet (1% ornithine w/v administered in drinking water, denoted by shaded blue boxes; n = 5 mice/group, mean ± s.e.m.; multiple unpaired t-tests, FDR-adjusted with two-stage linear step-up). e, Exogenous ornithine provided in drinking water (1% w/v) to conventional mice fed standard diet does not influence serum lipocalin-2 levels (mean ± s.e.m., n = 5 mice/group).

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We wanted to know whether Cd ornithine metabolism could affect severity of host inflammation. In both gnotobiotic (Fig. 3e) and conventional mice (Fig. 3f), ∆oraSE induced significantly higher levels of serum lipocalin-2, a readout of systemic host inflammation, than WT, despite lower overall abundance (Fig. 3d) and independent of significant differences in toxin production (Extended Data Fig. 4d). Dietary ornithine supplementation itself did not alter toxin production (Extended Data Fig. 4d) or serum lipocalin-2 (Extended Data Fig. 4e), suggesting that altered physiology or metabolism of the ∆oraSE strain itself influences host inflammatory response and the ability to metabolize ornithine by WT Cd results in less inflammation.

In mammals, ornithine plays a role in immunometabolism; arginase breaks arginine down to ornithine, which is a precursor for the polyamines spermidine, spermine and putrescine (Extended Data Fig. 5a)^33–36. Nitric oxide synthase also uses arginine as a substrate to generate NO⁻ during a reactive oxygen burst. Arginase activity has been described as the hallmark of a type-2-like inflammatory response as opposed to type 1, especially in macrophages³⁴.

Extended Data Fig. 5. Host ornithine metabolism via inducible nitric oxide synthase.

a, Intersecting host pathways act on arginine, producing nitric oxide (NO⁻) during an inflammatory response or ornithine via arginase during homeostatic or tissue-repair conditions. b, The ratio of ornithine to arginine in feces of conventional mice infected with WT Cd 630 (mean ± s.e.m., n = 5 mice/group; one-way ANOVA with Tukey’s post hoc comparisons: F_{(2, 12)}=4.742). c, Fecal ratio of ornithine to arginine in Cd-infected (R20291 WT or R20291 Tox-) mice fed standard diet depends on host iNOS genotype (mean ± s.e.m., n = 5 mice/group; one-way ANOVA with Tukey’s post hoc comparisons: F_(3,16)=8.614). Total Cd burden (d) and serum lipocalin-2 levels (e) in iNOS^−/− or iNOS^+/+ mice fed a fully defined ornithine-free diet co-infected with WT and ∆oraSE. For d and e, mean ± s.e.m. shown; n = 5 mice/group.

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We hypothesized that, in the absence of toxin-induced inflammation (Tox⁻), Cd may utilize host-derived ornithine. To determine whether host inducible nitric oxide synthase (iNOS) or arginase responds to CDI, we infected conventional mice with a standard antibiotic pretreatment (1 mg clindamycin per mouse by gavage) and subsequently infected with WT Cd. Compared to uninfected antibiotic-treated controls, mice infected with WT Cd suffered significantly higher tissue damage and inflammatory cell infiltrates quantified by blinded histopathological scoring (Fig. 4a). Host Nos2, but not Arg1, was upregulated concomitantly (Fig. 4b), suggesting that less arginine was available for ornithine biosynthesis under these conditions. Consistent with higher iNOS activity (increased NO⁻ generation), the ratio of ornithine to arginine in feces decreased from day 2 to 7 after infection (Extended Data Fig. 5b).

Fig. 4. Ornithine produced via host iNOS-deficiency provides competitive advantage to Cd in the gut lumen.

a, Blinded histopathology scoring of conventional mice fed standard diet infected with 630 WT 2 d and 7 d after infection (n = 5 mice per group) compared to antibiotic-treated controls (n = 4 mice, 2 d; n = 3 mice, 7 d; ANOVA F_(3,12) = 18.87 with Tukey’s post hoc comparisons; mean ± s.e.m.). b, iNOS expression in proximal colon tissue of mice infected with 630 WT is significantly higher 7 d after infection compared to 2 d and to uninfected, antibiotic-treated controls (WT day 7 significantly different from all other groups, ANOVA F_(3,11) = 12.97 with Tukey’s post hoc comparisons). Arginase-1 expression in the proximal colon tissue of mice is not significantly different between infected and uninfected mice. Expression levels are normalized to the antibiotic control group at day 2 (mean ± s.e.m., group sizes as in a). c, Genetic ablation of iNOS in mice fed standard diet leads to increased ornithine in feces during CDI (one-way ANOVA F_{(3, 16)} = 15.62 with Tukey’s post hoc comparisons; mean ± s.e.m., n = 5 mice per group). d, Increased expression of Cd ornithine aminomutase in iNOS^−/− mice infected with Tox⁻ Cd (expression levels normalized to WT in iNOS^+/+ mice, one-way ANOVA F_(3,15) = 5.795 with Tukey’s multiple comparisons. Mean ± s.e.m., n = 5 mice per group except for WT-infected iNOS^−/− mice, where two outliers were removed with robust regression and outlier removal implemented in Prism 9 (ROUT, Q = 0.1)). e, WT Cd outcompetes ∆oraSE in conventional iNOS^−/− but not iNOS^+/+ mice fed a fully defined ornithine-free diet (multiple unpaired Student’s t-tests with Benjamini, Krieger and Yekutieli two-stage step-up FDR correction; mean ± s.e.m., n = 5 mice per group).

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To increase the availability of arginine for ornithine biosynthesis via host arginase, we infected Nos2 knockout mice. In the absence of host iNOS activity (iNOS^−/−), the ratio of ornithine to arginine in feces was elevated during CDI compared to iNOS^+/+ mice (Extended Data Fig. 5c), in alignment with increased iNOS activity limiting ornithine production during inflammation. The concentration of ornithine in feces is elevated in iNOS^−/− compared to iNOS^+/+ mice (Fig. 4c) and Cd concurrently upregulated the d-ornithine aminomutase gene, oraE (Fig. 4d). In separate (WT or Tox⁻) infections, we could not rule out potential alterations in host immune response due to the Nos2 genotype. To disentangle the specific metabolic interaction between host iNOS and the Cd oxidative ornithine pathway, we co-infected iNOS^−/− and iNOS^+/+ mice on a fully defined ornithine-free diet with equal amounts of WT and ∆oraSE Cd. In alignment with our hypothesis that Cd utilizes host-derived ornithine, WT Cd outcompeted ∆oraSE in iNOS^−/− but not iNOS^+/+ mice (Fig. 4e). Nos2 genotype did not affect Cd abundance after initial colonization (Extended Data Fig. 5d) nor systemic inflammation (Extended Data Fig. 5e). Thus, increasing host ornithine production via iNOS ablation led to increased expression of Cd oxidative ornithine utilization genes and provided a competitive advantage to WT Cd.

Here, we investigate Cd persistence in the presence of commensal community members in the absence of symptoms of disease. It has been demonstrated previously that enteric pathogens benefit from physiological changes imparted by their virulence factors^37–40; however, the mechanisms by which Cd establishes asymptomatic carriage remain understudied.

We observed reduction of community amino acid metabolism in humanized mice that were susceptible to CDI and had elevated inflammation. Community pathways involved in ornithine, arginine and putrescine metabolism were reduced in Cd-susceptible mice before infection and absent after infection. Expression of oxidative ornithine metabolism unified Cd transcriptional expression in the absence of toxin production across two Cd strains and commensal communities. We previously reported that WT Cd exploits a metabolic product of the host inflammatory response¹⁰. Here, we demonstrate that it is possible for Cd to benefit from other host-derived compounds that are either non-inflammatory or are products of a type-2-like immune response. Our data are consistent with a recent study that conducted immunological transcriptional profiling of mouse cecal tissue in response to WT or non-toxigenic Cd¹¹ and found increased Nos2 expression but not Arg1 in response to WT infection. Furthermore, Cd ornithine aminomutase was more highly expressed by a tcdR mutant (alternative sigma factor that induces toxin expression) compared to WT and expression of the ornithine utilization locus in WT Cd decreased when inflammation was higher in vivo¹¹.

While Stickland metabolism-based ornithine metabolism has been investigated in other Clostridia, it had not been investigated in Cd, nor its implications investigated in vivo. We demonstrate that the ability to oxidatively degrade ornithine confers a competitive advantage due to the presence of dietary ornithine or host production of ornithine. This work highlights the metabolic cross-talk between host immunometabolism and Cd under homeostatic conditions in the gut. Understanding persistence of pathogens in the absence of disease is an important step toward preventative therapeutic strategies.

Methods

Mouse strains and husbandry

All animal experiments were performed in accordance with the Stanford Institutional Animal Care and Use Committee. For gnotobiotic mouse experiments, Swiss–Webster germ-free mice were maintained in gnotobiotic isolators. All animals were sex and age-matched and experiments were performed between 10 and 15 weeks of age. For conventional mouse experiments, Swiss–Webster Excluded Flora mice were used. For iNOS^−/− experiments, B6.129P2-Nos2^tm1Lau/J mice from The Jackson Laboratory were ordered and C57BL/6J WT mice were used as a comparison. Mice were fed an autoclaved standard diet (Purina LabDiet 5010 for conventional, 5K67 for gnotobiotic) except in experiments where a fully defined diet lacking ornithine (BioServ Product, S6185) was used (noted in text and figure legends).

Bacterial strains and growth conditions

Cd, B. thetaiotaomicron VPI-5482, C. sporogenes ATCC 35704, C. scindens ATCC 35704, Clostridium bolteae WAL-14578 and Bifidobacterium longum subspecies infantis ATCC 15697 strains were cultured anaerobically at 37 °C (85% N₂, 10% CO₂, 5% H₂). E. coli K-12 MG1655 and E. tarda ATCC 23685 were grown aerobically at 37 °C in LB medium with 10 µg ml⁻¹ vancomycin in plates. Solid medium used for Cd 630∆Erm and R20291 strains was CDMN (Cd agar base supplemented with 7% defibrinated horse blood, 0.5 mg ml⁻¹ cysteine, 32 µg ml⁻¹ moxalactam and 12 µg ml⁻¹ norfloxacin) and CDDC (Cd agar base (Oxoid) supplemented with 7% defibrinated horse blood, 0.5 mg ml⁻¹ cysteine, 250 µg ml⁻¹ d-cycloserine and 16 µg ml⁻¹ cefoxitin) for selective culture from mice and BHIS (Brain Heart Infusion Agar (Becton Dickinson) supplemented with 5 mg ml⁻¹ yeast extract and 0.5 mg ml⁻¹ cysteine) for routine culture from 25% (v/v) anaerobic glycerol stocks kept in glass crimp-top vials. Liquid medium used was BHIS (Brain Heart Infusion broth (BD) supplemented with yeast extract and 1 mg ml⁻¹ cysteine. Fully defined minimal medium for growth curves and in vitro competition experiments was CDDM without glucose (Cd defined medium) and modified BDM (basal defined medium⁴¹). B. thetaiotaomicron was cultured on BHI Blood Agar plates (Brain Heart Infusion (BD) supplemented with 10% defibrinated horse blood and 200 µg ml⁻¹ gentamycin; liquid medium used was BHIS for Bacteroides (Brain Heart Infusion agar (BD) supplemented with 5 µg ml⁻¹ hemin and 2 µg ml⁻¹ vitamin K1). TYG plates or broth were used for C. sporogenes culture (30 g l⁻¹ tryptone, 20 g l⁻¹ yeast extract, 1 g l⁻¹ sodium thioglycolate; plates were supplemented with 125 µg ml⁻¹ d-cyloserine, 38 µg ml⁻¹ sulfamethoxazole and 2 µg ml⁻¹ trimethoprim). C. scindens was cultured on CDMN plates and in TYG broth. B. infantis was cultured on de Man, Rogosa and Sharpe (Sigma-Aldrich) solid medium supplemented with 0.25% (w/v) l-cysteine and in TYG broth. C. bolteae was cultured on solid Reinforced Clostridial Agar (Sigma) and in Reinforced Clostridial Medium (Becton Dickinson).

Gnotobiotic colonizations and pathogen infections

For experiments where transcriptional profiling and untargeted mass spectrometry was performed to compare WT and Tox⁻ infections versus uninfected control groups, germ-free mice were gavaged with 200 µl of a 1:1:1 mixture of the three community members (DC1, B. thetaiotaomicron, C. sporogenes and E. coli or DC2, B. thetaiotaomicron, C. scindens and E. tarda). Consortia were allowed to stabilize for 10–15 d in mice before introduction of Cd or were gavaged with anaerobic PBS for the uninfected control group. For experiments to determine the influence of dietary ornithine availability on longitudinal toxin production, germ-free mice were gavaged with 200 µl mixture of volumetrically equal parts B. thetaiotaomicron, E. coli, C. bolteae and B. infantis. The community stabilized for 13 d before Cd colonization.

In all gnotobiotic experiments, no antibiotic pretreatment was used before infection. Fourteen-hour Cd cultures grown in BHIS were fogged into isolator ports in 2-ml inner-threaded cryovials 2 h before infection such that mice were gavaged with 200 µl 16-h Cd culture grown in BHIS, corresponding to ~1 × 10⁸ c.f.u. ml⁻¹.

For conventional mouse experiments, mice were gavaged with 1 mg clindamycin in 200 µl water 24 h before infection with 200 µl Cd grown in BHIS medium for 16 h. For co-infections, a 1:1 mixture of each Cd strain was used. If mice were fed an ornithine-free diet, diet was switched 24 h before antibiotic pretreatment (48 h before Cd infection). Bedding was replaced after diet switch, clindamycin gavage and after Cd infections.

Generation of Cd mutants

The Cd 630∆Erm∆pyrE∆oraSE, ∆argF and ∆argM mutants were constructed via the pyrE allelic exchange system as previously described⁴². Briefly, regions 1 kb upstream and downstream of the targeted deletion region were amplified from purified WT genomic DNA (Supplementary Table 4) and inserted into the pMTL-YN3 vector backbone via Gibson Assembly, transformed into and propagated in E. coli TG1 before transformation into E. coli HB101/pRK24 conjugation-proficient cells. The pMTL-YN3 deletion plasmid was transferred to Cd 630∆Erm∆pyrE via spot-plate mating conjugation. During conjugation and subsequent selection steps, Cd and E. coli were cultured anaerobically with higher atmospheric H₂ atmospheric levels: 85% N₂, 5% CO₂ and 10% H₂. Plasmid integrants were selected for on BHIS medium supplemented with 15 µg ml⁻¹ thiamphenicol, 50 µg ml⁻¹ kanamycin, 16 µg ml⁻¹ cefoxitin and 5 µg ml⁻¹ uracil, and deletion mutants on CDDM supplemented with 5 µg ml⁻¹ uracil and 2 mg ml⁻¹ 5-fluoroorotic acid. Deletion loci were sequence-verified, after which the pyrE locus was restored to the ∆oraSE mutant with a second round of mutagenesis using the pMTL-YN1C plasmid.

Histopathology scoring

Tissue segments of approximately 1 cm in length were excised from the cecal blind tip and most proximal colonic section of mice with a clean razor blade, placed in plastic cassettes, fixed in formalin for 24–48 h, then transferred to 70% ethanol for long-term storage. Tissues were embedded in paraffin, sectioned and stained with hematoxylin and eosin. Tissues (cecal blind tip and proximal colon) were given scores of 0–3 in each of the following parameters: inflammatory cell infiltration, mucosal hyperplasia, vascular congestion, epithelial disruption and submucosal edema. The cumulative lesion score is the sum of each score in these independent categories. Scoring was performed blinded.

16S sequencing and analysis

Total DNA was extracted from frozen fecal samples using the DNeasy PowerSoil HTP 96 kit (QIAGEN). Barcoded primers were used to amplify the V3–V4 region of the 16S rRNA gene using 515f and 806r primers. The Ultra Clean 96 well PCR Clean-Up kit (QIAGEN) was used to clean the amplicons before quantification of DNA yield (Quant-iT), library pooling and quality control with BioAnalyzer. The 250–300-bp paired-end reads were generated on Illumina MiSeq2500. Demultiplexing was performed with Qiime 1.9 (ref. ⁴³) and reads were assigned to a custom 16S rRNA reference database of the defined communities using pick_closed_reference_otus.py.

RNA-seq and analysis

RNA isolation

RNA from cecal contents was isolated using phenol:chloroform extraction followed by cleanup with the RNEasy Mini kit (QIAGEN) or using the RNA Power Microbiome kit (QIAGEN) per the manufacturer’s instructions.

Library prep and sequencing

Ribosomal RNA was depleted from total RNA isolated from cecal contents using the Illumina Ribo-Zero Gold Epidemiology rRNA Removal kit. Depletion of rRNA and RNA quality was verified with Agilent Bioanalyzer Prokaryote Total RNA Pico before moving forward with library preparation using the Illumina TruSeq mRNA Stranded HT Library Prep kit. Sequencing for in vivo RNA-seq was performed on an Illumina HiSeq4000 instrument with 100-bp paired-end reads.

Analysis

Raw, paired reads were imported into CLC Genomics Workbench v.11 with a maximum distance of 1,000 bp. In vivo reads were trimmed with a quality limit of 0.05, an ambiguous limit of 2, based on automatic read-through adaptor trimming, with a minimum number of nucleotides of 50. Broken pairs and discarded sequences were not saved. Reads were mapped to the following reference genomes: Cd 630 genome (RefSeq accession NC_009089.1), Cd R20291 (RefSeq, FN545816.1), B. thetaiotaomicron VPI-5482 (RefSeq, NC_004663.1), E. coli str. K-12 substr. MG1655 (RefSeq, NC_000913.3), C. scindens ATCC 35704 (RefSeq, NZ_CP036170.1), E. tarda ATCC 23685 (RefSeq, NZ_ADGK00000000.1) and C. sporogenes ATCC 15579 (RefSeq, NZ_ABKW00000000.2). Standard parameters were used; paired reads were mapped to the gene track with a mismatch cost of 2, insertion cost of 3, deletion cost of 3 and length and similarity fractions of 0.8. Strand specificity was used; paired distances were auto-detected and reads that mapped in pairs were counted as one hit. A maximum of ten hits per read was allowed. Total read counts for each gene were exported and remaining ribosomal RNA reads were manually removed before normalization and differential expression analysis in R with DESeq2 (ref. ⁴⁴).

For differential expression analysis, comparable experimental conditions (same defined community, same sequencing run) were combined into one DESeq object to be fitted with a negative binomial GLM. Low-count reads were filtered at a count threshold of eight for 630 and five for R20291. Pairwise contrasts were specified between the experimental groups to define significance with the Wald test.

RT–qPCR

RNA from frozen host proximal colon tissue was extracted with the RNEasy Mini kit; 25–30 mg tissue was bead-beat with acid-washed glass beads in 600 µl RLT + ßME for 5 min at 4 °C before proceeding with the kit protocol. Complementary DNA was generated using random primers and Superscript III Reverse Transcriptase per the manufacturer’s instructions; RNase OUT was included. Quantitative PCR (qPCR) was performed with SYBR Brilliant III on an Agilent MX3000P or CFX Connect Real-Time System in 20-µl volumes with 0.3 µl reference dye added. Changes to expression were calculated by normalizing Ct value to Gapdh (host tissue) or rpoA (Cd expression) with the Δ-Δ Ct method.

Competition assays

In vivo and in vitro competition between WT and mutant strains was assessed using qPCR. Primer pairs (Supplementary Table 4) targeting unique loci within the WT oraE, argF or argM genes (WT) or surrounding their respective deletions were validated with serial dilutions of purified genomic DNA. Efficiency values for each primer pair were calculated as 10^(1/-slope) of log₁₀(DNA input) against the Ct value. For in vivo competition, total DNA was extracted from frozen fecal samples of conventional mice co-infected with WT and ∆oraSE Cd using the DNeasy PowerLyzer PowerSoil kit (QIAGEN). For in vitro competitions, 5-ml cultures were co-inoculated with 1:100 diluted combined (1:200 dilution each) overnight culture of WT and a mutant strain. After 24 h growth, cells were pelleted and total DNA was extracted by modification of existing protocols⁴⁵ as follows: one wash with 1× TE, incubation in 50 µl 50 mg ml^–1 lysozyme and 200 µl genomic DNA solution for 30 min at 37 °C, 100 µl 20% Sarkosyl and 15 µl 10 mg ml^–1 RNase A for 30 min at 37 °C, 7.5 µl 20 mg ml^–1 proteinase K and 200 µl buffer AL (QIAGEN) for 30 min at 56 °C, addition of 200 µl 100% ethanol before proceeding with the DNeasy Blood and Tissue kit (QIAGEN). qPCR reactions were performed as described above. The competitive index of each genotype was calculated as 2^−Ct WT primer pair / 2^−Ct mutant primer pair. A competitive index of 1 indicates that each strain is at equal abundance.

Toxin B quantification

The tgcBIOMICS Toxin B ELISA kit (TGC-E002-1) was used to measure Cd TcdB concentration in mouse feces or cecal contents with anti-tcdB-HRP. The 25–50 mg frozen fecal samples were thawed, weighed and resuspended thoroughly in 450 µl dilution buffer. Resuspended fecal samples were allowed to sediment and 100 µl supernatant was used in the assay, proceeding via the manufacturer’s instructions. Toxin levels were quantified (ng toxin per g feces) using a 1:2 dilution series standard curve in duplicate (40 ng ml^–1 to 0.31 ng ml^–1) and normalized to the absolute abundance of Cd levels (c.f.u. ml^–1) from the same mouse, as previously described¹⁰.

Lipocalin-2 quantification

Mouse serum lipocalin-2 levels were measured using the Mouse Lipocalin-2/NGAL DuoSet ELISA kit (R&D Biosystems). Mouse serum previously snap-frozen in liquid nitrogen and stored at −80 °C was thawed on wet ice, mixed and diluted 1:250 and 1:1,000 in 1× PBS containing protease inhibitor (Roche cOmplete Mini Protease Inhibitor Cocktail) before proceeding via the manufacturer’s instructions. Quantification was performed with a ten-point standard curve in triplicate.

Mass spectrometry assays

Untargeted quantification of cecal metabolites

Germ-free Swiss–Webster mice harboring a defined community of bacteria (B. thetaiotaomicron, C. sporogenes and E. coli) were infected with Cd WT R20291, R20291 Tox⁻ or gavaged with anaerobic PBS as a control. After 5 d of infection, cecal contents were collected and flash-frozen in liquid nitrogen. Cecal contents were weighed to ~10 mg and shipped on dry ice to the West Coast Metabolomics Center at University of California, Davis. For the WT-infected group, two fecal samples were included due to limited volume of cecal contents in the hypervirulent infection, which were also used for RNA-seq. Then, 4 ± 0.3 mg samples were extracted, derivatized and analyzed by gas chromatography time of flight mass spectrometry (GC–TOF–MS) as described previously⁴⁶. Reported data (Supplementary Table 1) represent peak heights for each ion normalized to the sum of the peak heights (mTIC) for all named metabolites within a treatment group. Identified metabolites (n = 220) were included in downstream analysis. Peak areas were log-transformed and statistical differences between groups was performed with Metaboanalyst⁴⁷.

Targeted quantification of amino acids

We modified an existing protocol⁴⁸ to quantify amino acids from complex biological matrices. Feces were thawed and weighed before metabolite extraction. Fecal samples were subsequently resuspended in 1:1 LC–MS-grade acetonitrile:water, beat with acid-washed glass beads for 5 min at 4 °C, incubated for 5 min at room temperature and centrifuged for 5 min. Then, 50 µl supernatant was mixed with 25 µl 0.1 M sodium tetraborate and 50 µl dansyl chloride (50 mM in 100% acetonitrile). Samples were incubated for 30 min at room temperature in the dark, mixed at the 15-min interval. Then, 50 µl 0.5% formic acid was added to stop the derivatization reaction and samples were subsequently filtered through a 0.45-µm low-binding filter plate (Millipore). A 5-µl sample was injected onto a 10-cm C18 column (Acquity) with a flow rate of 0.5 ml min^–1 in 0.1% formic acid in water (solvent A) and 0.1% formic acid in acetonitrile (solvent B). Solvent A started at 95% and B at 5%; from 0–10.25 min, A decreased to 40%; 10.25–12.5 min, A decreased to 5%; 12.5–15 min A held at 5%; 15–15.1 min A increased to 95% and held until the end of the run at 18 min. Column temperature was held at 40 °C throughout. Metabolites were detected with an Agilent 6460 Triple Quad mass spectrometer in positive ionization mode using 300.11 (l-ornithine), 303.63 (d₇-l-ornithine) and 408.17 (l-arginine) precursor ions, with 171 as the product. A 100 [ms] dwell time was used for l-arginine and 150 [ms] for l-ornithine; 120 [V] fragmentor voltage for l-arginine and 100 [V] for l-ornithine; 40 [V] collision energy for l-arginine and 10 [V] for l-ornithine. Peaks were identified and integrated with Agilent MassHunter Workstation Software Quantitative Analysis. External standard curves of l-ornithine were generated and d₇-l-ornithine was used as an internal standard during protocol development.

Analysis of previous datasets

Human microbial communities classified as dysbiotic or healthy-like are described in our previous work¹³. In brief, 16S rRNA sequencing was performed on samples provided by healthy human donors (n = 118) and samples from patients presenting with diarrhea (n = 115). Microbial composition separated patients into two clusters: dysbiotic (lower α- and β-diversity) versus healthy. Partitioning around medoids, the gap statistic and average silhouette width clustering analyses were used to categorize samples; all healthy donors fell into one category (healthy-like), whereas the samples provided by patients with diarrhea were separated into healthy-like and dysbiotic. A subset (six each from the healthy-like and dysbiotic groups) of these human samples were chosen for mouse humanization and subsequent challenge with Cd. RNA-seq was performed on fecal pellets before and after Cd infection; gene and pathway expression profiles were obtained with HUMAnN2 v.0.5.0. RNA-seq results were normalized to shallow metagenomic reads; pathway differential expression analysis was performed with DESeq2 v.1.8.2 and LEfSe.

Published microarray data³² are available through the Gene Expression Omnibus under the accession number GSE60751. Analysis comparing the two Cd-monocolonized groups (standard diet versus polysaccharide-deficient diet) was performed in GEO2R with standard analysis parameters.

Reporting Summary

Further information on research design is available in the Nature Research Reporting Summary linked to this article.

Source data

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Author contributions

K.M.P and J.L.S. conceived the project, designed experiments and wrote the manuscript. K.M.P. designed and performed experiments and analyzed data. E.B. and P.K. contributed data. O.R.D. and M.D. assisted with bacterial mutagenesis. S.K.H. and F.E. assisted with animal experiments. D.D., C.F., A.J.H. and W.V.T. assisted with mass spectrometry and bacterial culture protocol development.

Data availability

Raw RNA-seq data are available at NCBI SRA with accession no. PRJNA687238; untargeted GC–TOF metabolomics data are available at Metabolomics Workbench (https://www.metabolomicsworkbench.org/) under accession no. ST001650.

Code availability

Code is available at https://github.com/kpruss/Cdiff-ornithine. Source data are provided with this paper.

Competing interests

The authors declare no competing interests.

Extended data

is available for this paper at 10.1038/s42255-021-00506-4.

Supplementary information

The online version contains supplementary material available at 10.1038/s42255-021-00506-4.