Skip to main content
. 2021 Jul 29;107(2):478–488. doi: 10.3324/haematol.2021.278530

Figure 5.

Figure 5.

Ndfip1 regulates ferroportin in vivo. (A) Green fluorescent protein (GFP) was detected in the liver of AAV2/8-shControl- and AAV2/8-shNdfip1-injected mice. GAPDH was used as a loading control. (B) AAV2/8-shNdfip1 reduced the level of Ndfip1 mRNA in the liver, as determined by quantitative polymerase chain reaction (qPCR) (mRNA expression relative to control; **P<0.01; Student’s t-test). (C) The level of ferroportin (FPN) in the liver of AAV2/8-shNdfip1-treated mice was increased compared to AAV2/8-shControl-treated mice, as determined by immunoblot. GAPDH was used as a loading control. (D) Densitometric analysis of immunoblot in (C) (***P<0.001; Student’s t-test). (E) HAMP mRNA expression was similar in the liver of AAV2/8-shNdfip1-treated mice compared to AAV2/8-shControl-treated mice as determined by qPCR (mRNA expression relative to control). (F) Serum hepcidin levels were similar in AAV2/8-shNdfip1- and AAV2/8-shControl-treated mice as determined by enzyme-linked immunosorbant assay. (G) Serum iron levels were increased in mice treated with AAV2/8-shNdfip1 (*P<0.05; Student’s t-test).