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. 2021 Jul 27;13(2 Suppl):1467S–1477S. doi: 10.1177/19476035211023550

Figure 2.

Figure 2.

MiR-146a-5p negatively regulated NUMB expression via a direct binding site at 3′UTR of NUMB mRNA. (A) There is a potential miR-146a-5p binding sequence in the 3′UTR of NUMB mRNA. (B) The relative luciferase activity after the co-transfection of miR-146a-5p mimic/inhibitor and 3′UTR of NUMB mRNA. (C, D) QRT-PCR showed the miR-146a-5p and NUMB mRNA levels in the 2 types of cells after transfection with miR-146a-5p mimic or inhibitor. (E, F) Western blotting showed the NUMB protein levels in the 2 types of cells after transfection with miR-146a-5p mimic or inhibitor.