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. 2022 Jan 3;13(1):370–382. doi: 10.1080/21655979.2021.2005743

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — MiR-18a-3p is associated with OP and osteogenic differentiation (a) miR-18a-3p expression level in OP-no-Frt and OP-Frt was determined through qRT-PCR. **P < 0.001. (b) miR-18a-3p expression level in hBMSCs osteogenesis was detected via qRT-PCR. (c) ALP activity of cells described in hBMSCs osteogenesis. (d) Osteogenesis related genes ALP was detected in hBMSCs osteogenesis by qRT-PCR. (e) ARS was applied to quantify the bone mineralization ability of hBMSCs. (f-h) Osteogenesis related genes RUNX2 (f), OCN (g) and OPN (h) were detected in hBMSCs osteogenesis by qRT-PCR. (i). ELISA analysis of OCN expression in culture medium at different time. (j) Western blotting analysis demonstrating Collagen I and II expression in hBMSCs osteogenesis.*P < 0.05 vs. 0 day; **P < 0.001 vs. 0 day.