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. 2022 Jan 3;13(1):370–382. doi: 10.1080/21655979.2021.2005743

Figure 2.

Figure 2.

The low expression of miR-18a-3p promotes osteogenic differentiation, while overexpression of miR-18a-3p inhibits osteogenic differentiation. (a) miR-18a-3p level was determined by qRT-PCR in hBMSCs transfected with NC, miR-18a-3p inhibitor and miR-18a-3p mimic. (b) Cell viability of hBMSCs transfected with NC, miR-18a-3p inhibitor and miR-18a-3p mimic was measured by CCK-8 assay. (c) ALP activity of cells described in hBMSCs transfected with NC, miR-18a-3p inhibitor and miR-18a-3p mimic. (d) Osteogenesis related genes ALP was detected in hBMSCs transfected with NC, miR-18a-3p inhibitor and miR-18a-3p mimic by qRT-PCR. (e) ARS was applied to quantify the bone mineralization ability of hBMSCs transfected with NC, miR-18a-3p inhibitor and miR-18a-3p mimic. (f-h) Osteogenesis related genes RUNX2 (f), OCN (g) and OPN (h) were detected in hBMSCs transfected with NC, miR-18a-3p inhibitor and miR-18a-3p mimic by qRT-PCR. (i) ELISA analysis of OCN expression in culture medium of different groups. (j). Western blotting analysis demonstrating Collagen I and II expression in hBMSCs osteogenesis in different groups. *P < 0.05, **P < 0.001 vs. blank.