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. 2022 Jan 3;13(1):370–382. doi: 10.1080/21655979.2021.2005743

Figure 4.

Figure 4.

miR-18a-3p controls osteogenic differentiation through GRIA1. (a) GRIA1 expression level was determined by qRT-PCR in hBMSCs transfected with miR-18a-3p mimic or OE-GRIA1. (b) GRIA1 expression level was determined by Western blot in hBMSCs transfected with miR-18a-3p mimic or OE-GRIA1. (c) Cell viability of hBMSCs transfected with miR-18a-3p mimic or OE-GRIA1 was measured by CCK-8 assay. (d) ALP activity of cells described in hBMSCs transfected with miR-18a-3p mimic or OE-GRIA1. (e) Osteogenesis related genes ALP was detected in hBMSCs transfected with miR-18a-3p mimic or OE-GRIA1 by qRT-PCR. (f) ARS was applied to quantify the bone mineralization ability of hBMSCs transfected with miR-18a-3p mimic or OE-GRIA1. (g-i) Osteogenesis related genes RUNX2 (g), OCN (h) and OPN (i) were detected in hBMSCs transfected with miR-18a-3p mimic or OE-GRIA1 by qRT-PCR. (j). the osteogenic marker OCN was detected by ELISA. (k). Western blotting analysis demonstrating Collagen I and II expression in hBMSCs osteogenesis in different groups. *P < 0.05, **P < 0.001 vs. blank.##P < 0.05, ##P < 0.001 vs. mimic + OE.