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. 2022 Jan 3;13(1):810–823. doi: 10.1080/21655979.2021.2012952

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The interaction relationship of circPTK2 and miR-136-5p. (a and b) circPTK2 enrichment level was detected after Ago2 or IgG RIP assay in HT-29 and LoVo cells. (c) The targeted miRNAs of circPTK2 were predicted and analyzed via starBase and circinteractome. (d) The targeted miRNA levels in CRC were predicted via dbDEMC 2.0. (e and f) miRNA levels were measured after RNA pull-down analysis. (g) The binding site of circPTK2 and miR-136-5p. (h) miR-136-5p level was determined in HT-29 and LoVo cells with transfection of miR-NC or miR-136-5p mimic. (i and j) Luciferase activity was measured in HT-29 and LoVo cells with transfection of circPTK2-wt or circPTK2-mut and miR-NC or miR-136-5p mimic. (k) miR-136-5p expression was measured in CRC and normal samples. (l) miR-136-5p abundance was examined in HT-29, LoVo and NCM460 cells. *P < 0.05, **P < 0.01, ***P < 0.001.