Figure 6.

The blocking role of miR-188-3p on the function of germacrone by mediating podocyte apoptosis. (a) Micrographs of kidney sections in WT, STZ-induced mice, STZ+germacrone co-treated mice, STZ+germacrone+miR-188-3p agomir co-induced mice and STZ+germacrone+miR-188-3p agomir NC co-treated mice were recorded by electron microscopy; the red arrows point to the damaged mitochondria. Scale bar: 1 μm. Magnification: ×7000. (b) Mitochondrial ROS production was evaluated in the above groups with a DCFH-DA ROS Assay kit, **P < 0.01. (c)-(d) RNA level of miR-188-3p and GPX4, podocin, and nephrin proteins were measured in different groups, **P < 0.01. (e) Quantitative analysis of protein expression including GPX4, podocin, and nephrin. **P < 0.01.