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. 2021 May 23;12(1):1952–1963. doi: 10.1080/21655979.2021.1921553

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Overexpression of circ_0062582 promoted the osteogenic differentiation of hBMSCs. (a) Expression levels of circ_0062582 in hBMSCs was detected by RT-qPCR analysis. (b) Detection of the expression of circ_0062582 following addition of pcDNA-circ_0062582. (c) Viability of hBMSCs was measured using CCK-8 assay. (d) Alkaline phosphatase staining was used to detect the differentiation activity of hBMSCs. Magnification, x200. (e) Alizarin red staining was used to detect the mineralization levels of hBMSCs. Magnification, x200. (f) Protein expression levels of OSX, OCN and COL1 were tested by means of western blot assay. **P < 0.01 and ***P < 0.001. hBMSCs, human bone marrow mesenchymal stem cells; circ, circular RNA; OSX, osterix; OCN, osteocalcin; COL1, collagen type 1