Figure 5.

Role of STAT3-FTO in the doxorubicin-induced apoptosis of MCF-7 cells. Doxorubicin-resistant MCF-7 or MCF-7 cells were transfected with FTO siRNAs or FTO overexpression plasmids and co-transfected with FTO siRNA and FTO overexpression plasmids for 48 h and then exposed to doxorubicin for 24 h. a, b and d Apoptotic MCF-7 cells were detected by Annexin V/PI staining. c, e Expressions of cleaved PARP and cleaved caspase-3 in doxorubicin-resistant MCF-7 and MCF-7 cells. **p < 0.01. MCF-7-DoxR, doxorubicin-resistant MCF-7; siNC, negative control siRNA; siFTO, FTO siRNA; NC-OE, empty vector; OE-FTO, FTO overexpression; PI, propidium iodide; PARP, poly (ADP-ribose) polymerase