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Differentiation of hBM-MSCs into osteoblasts. (a) Alizarin Red staining of hBM-MSCs shows a large number of calcified nodules in 14 days. (b) Relative mRNA expression of ALP and RUNX2 during osteogenic differentiation of hBM-MSCs determined by qRT-PCR. ns, not significant relative to D0 group, P > 0.05; *P < 0.05; **P < 0.01
