Figure 5.

Dual luciferase reporter detection of target genes of miR-4739. (a)Detailed annotation map of hsa_circ_0006766 with miR-4739. Only the binding sites of the coding sequence in hsa_circ_0006766 are shown.(b) Predicted binding sites of miR-4739 in Notch2, ZFP36L1, and Wnt1 determined by TargetScan. Relative luciferase activities in cells overexpressing miR-4739 with wild-type or mutated miR-4739 binding sites in (c) Notch2, (d) ZFP36L1, and (e) Wnt1. ns, not significant, P > 0.05; ** P < 0.01; ****P < 0.0001