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. 2021 Sep 21;12(1):7177–7193. doi: 10.1080/21655979.2021.1979442

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — APCDD1L-AS1 confers resistance to 5-FU in OSCC cells

(A) RT-qPCR was performed to measure APCDD1L-AS1 level in HSC-3/5-FU and HN-4/5-FU cells transfected with sh-NC and sh-APCDD1L-AS1. (B) CCK-8 was performed to assess IC50 of 5-FU in HSC-3/5-FU and HN-4/5-FU cells transfected with sh-NC and sh-APCDD1L-AS1. (C) Colony formation assay was conducted to examine the cell viability of HSC-3/5-FU and HN-4/5-FU cells transfected with sh-NC and sh-APCDD1L-AS1. (D) Flow cytometry analysis was conducted to probe the apoptotic rate of HSC-3/5-FU and HN-4/5-FU cells transfected with sh-NC and sh-APCDD1L-AS1. **P < 0.01.