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. 2021 Sep 21;12(1):7177–7193. doi: 10.1080/21655979.2021.1979442

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — APCDD1L-AS1 is transferred extracellularly via exosome incorporation in 5-FU-Resistant OSCC Cells

(A) APCDD1L-AS1 expression was detected by RT-qPCR after cells were treated with 1 μg/mL RNase A or 1 μg/mL RNase A + 0.1% Triton X100 for 30 min. (B) The exosomes images secreted by HSC-3/5-FU and HN-4/5-FU cells were showed by TEM scanning. (C) The levels of CD63 and CD9 proteins were measured by Western blot in HSC-3/5-FU and HN-4/5-FU cells. (D) APCDD1L-AS1 expression in exosomes of parental and 5-FU-sensitive NSCLC cells was detected by RT-qPCR. **P < 0.01.