Figure 13.

Rhein treatment affected ERs and autophagy of 3T3-L1 cells with inflammatory stimulation. Representative images and quantification of ERs-related proteins and autophagy-related protein in 3T3-L1 cells with inflammatory supernatant induction. The supernatant culturing Raw246.7 cells with LPS stimulation was collected and used to incubate with 3T3-L1 preadipocytes. Each experiment was repeated at least three times. ***p < 0.001 versus control. #p < 0.05, ##p < 0.01, ###p < 0.001 versus Model