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. 2021 Sep 21;12(1):6844–6854. doi: 10.1080/21655979.2021.1972076

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Effects of calcitriol on the functions of NK cells. (a) Analysis of the cytokine released by the calcitriol-treated NK cells in 48 h. (b) Fluorescence-activated cell sorting (FACS) results showed dead K562 cells labeled by 3,3′-dioctadecyl-oxacarbocyanine (DIO) and propidium iodide (PI). K562 cells were co-cultured with different ratios of calcitriol-treated NK cells for 4 h. (c) FACS analysis of dead K562 cells in NK-killing assays. (d) The degranulation effects of treated NK cells in killing assays. n = 3 for each group. *P < 0.05 and **P < 0.01 were compared with NC